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CISH Ancillaries

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  • لام شارژ شده / Positive Charged Slides
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    لام شارژ شده Positive Charged Slides

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  • دستگاه هیبریدایزر Hybridization System
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    دستگاه هیبریدایزر Hybridization System

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  • چسب فیکسوگام
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    چسب فیکسوگام Rubber Cement Fixogum

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    Rubber Cement Fixogum is an elastic adhesive, ideal for “in situ” hybridization techniques for sealing the
    coverslip on the slide once the probe is applied.

  • Cover Slip - لامل
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    Cover Slip – لامل

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  • اقلام مصرفی سیش

    TBS Tween 20 Buffer 10X

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    Product Composition: The composition of the 10x concentrated product is: Tris-HCl 500 mM, NaCl 3M, 0.5 % Tween 20, pH 7.5.

    Recommendations for Use:
    Before start using the product: This solution is 10x concentrated, so before its use it must be diluted
    1:10 with double-distilled or deionized water (1 part of buffer and 9 parts of water).

  • EDTA Buffer 10X pH8
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    EDTA Buffer 10X pH8

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    Product Composition: 10mM EDTA pH8.0.

    Intended Use: Product for in vitro diagnostic use

    Recommendations for Use:
    Before use: The EDTA solution is 10x concentrated and therefore before use it should be diluted in a 1:10 range with distilled or deionized water (one part EDTA buffer and 9 parts water).
    -Cut and mount sections on charged slides or silane coated.

    -Dewaxing (using xylene or substitute) and rehydrate the tissue sections thru decreasing series of alcohol. Then follow with the step 3 and 4.

    -Unmasking: various alternative methods could be used to carry out the antigen retrieval
    process using the diluted EDTA buffer

    -Remove the sections and place them into the usual washing buffer (recommended TBS / TBS-Tween pH 7.5 – reference MAD-004077R)

    -Proceed with the immunostaining protocol as usual.

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