مونوکلونال

.(Name: Glycophorin A  Antibody (Clone JC159

Description and aplications: This antibody reacts with a resistant paraffin-embedded and formol fixed epitope located in the extracellular domain of the protein, probably between amino acids 27 and 40. Therefore, this antibody recognizes normal erythroid cells in all stage of differentiation from erythroblasts to mature red blood cells. Once glycophorin A is at its maximum expression, the quantity in each erythroid cell remains constant and shows no change during maturation despite the decreasing size of the cell. The neoplastic erythroblast in most erythroleukemias are identified by Glycophorin A.

Composition: anti-Glycophorin A mouse monoclonal antibody obtained from supernatant culture and prediluted in a tris buffered solution pH 7.4 containing 0.375mM sodium azide solution as bacteriostatic and bactericidal.

Immunogen: membrane preparation from splenic hairy cell leukemia cells.

.(Name: Glypican 3 Antibody (Clone 1G12

Description and applications: Glypican‐3 (GPC3) is a member of the glypican family of glycosyl phosphatidylinositol‐anchored cell‐surface heparin sulfate proteoglycans. The 1G12 monoclonal antibody has been used to assess GPC3 expression in malignant and nonmalignant liver tissue samples and for enzyme‐linked immunosorbent assay (ELISA) for detection of GPC3 in the serum. Studies have shown that GPC3 is expressed at the protein level in most HCCs, but it is undetectable in normal liver and benign hepatic lesions, including dysplastic and cirrhotic nodules. In addition, GPC3 is significantly elevated in the serum of a large proportion of patients with HCC. Based on these results, it has been proposed that GPC3 could be a useful marker to differentiate between benign and malignant liver diseases. The GPC3 is expressed in the great majority of the gonadal yolk sac tumors. The endodermal component of endometrial or ovarian endometrioid carcinoma cases with also show intense cytoplasmic and / or membrane positivity. It is positive in sporadic cases of choriocarcinoma and presents focal positivity in embryonal carcinomas and immature teratomas; is not expressed in seminomas an intratubular germ cell neoplasia. Isolated cases spermatocytic seminomas show weak positivity. Other tumors of gonadal origin that can express GPC3 are endometrioid, clear cell, mucinous or serous carcinomas. 80% of placental site trophoblastic tumors and 100% of uterine placental site nodules are also positive. In other no gynecological tumors, GPC3 is expressed to 23% of lung carcinomas (55% squamous cell carcinomas and only 8% of adenocarcinomas) and 3% of renal cell carcinomas (chromophobe carcinomas). Along with other markers such as E-cadherin (negative) and β-catenin (positive nuclear and cytoplasmic), GPC3 is a useful marker in the diagnosis of pseudopapillary solid tumors of the pancreas and helps the differential diagnosis of neuroendocrine tumors. 34.7% of rhabdomyosarcomas, more frequent embryonal type, show positivity for this antibody while other types of sarcomas arenegative. In a recent study, 52% of liposarcomas and 29% of melanomas have been positive. Varying percentages of other tumors of epithelial and mesenchymal have shown variable positivity, demonstrating the lack of specificity of this antibody and the need to analyze the results in context with other antibodies staining.

Composition: anti-human Glypican 3 mouse monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Immunogen: Balb/C mice immunized with a single i.p. injection of 50 μg of a fragment containing the last 70 amino acids of the core protein

.(Name: Granzyme B Antibody (Clone GrB-7

Description and aplications: This antibody reacts with human Granzyme B. Granzyme B is a neutral serine protease which is stored in granules of specialized cytotoxic T lymphocytes (CD8) and NK cells. From mouse CD8 and NK cells vaious types of granzymes have been identifies and named alphabetically (A-G). They are involved in the apoptosis mediated by the cytotoxic lymphocytes. The exocytosis of granules containing granzyme in the cytoplasm of the target cells leads to DNA fragmentation and subsequent apoptosis. Granzyme B has also been located in EBV positive Reed Sternberg cells of Hodgkin’s disease.

Composition: anti-Granzyme B mouse monoclonal antibody obtained from supernatant culture and prediluted in a tris buffered solution pH 7.4 containing 0.375mM sodium azide solution as bacteriostatic and bactericidal.

Immunogen: Recombinant protein encoding the N-terminus of the mature granzyme B.

Name: Hepatocyte Specific Antigen (Hep Par 1) Antibody (Clone OCH1E5)

Description and applications: Anti-hepatocyte specific antigen, also known as anti-Hep-Par1, recognizes both benign and malignant liver-derived tissues including such tumors as hepatoblastoma, hepatocellular carcinoma (including its fibrolamellar variant), and hepatic adenoma. It recognizes both normal adult and fetal liver tissue. The typical pattern is a granular cytoplasmic staining. This antibody is useful in differentiating hepatocellular carcinomas with adenoid features from adenocarcinomas, either primary in the liver or metastatic lesions to the liver. In labeling hepatoblastoma, it is useful in differentiating this entity from other small round cell tumors. No reaction is usually seen with other human tumors with the exception of some gastrointestinal tumors. Occasionally it might be expressed by some tumors which include adrenal carcinomas, yolk sac tumors, adenocarcinomas of the colon, lung, ovarian carcinomas and adenocarcinomas of the endocervix.

Composition: anti-human HepPar 1 mouse monoclonal antibody purified from ascites. Prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

.(Name: TCR beta Antibody (Clone G-11

Description and applications: The T cell antigen receptor (TCR) recognizes foreign antigens and translates such recognition events into intracellular signals that elicit a change in the cell from a dormant to an activated state. TCR is a heterodimer composed of either α and β or γ and δ chains. The vast majority of circulating T cells (95%) express the α/β heterodimer while roughly 2-5% express the γ/δ heterodimer.

Composition: anti-human TCR beta mouse monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Immunogen: human constant region of T cell receptor beta.

Name: Rabbit Anti-c-erbB2 Monoclonal Antibody clone SP3

Description and applications: C-erbB-2 is a receptor tyrosine kinase of the c-erbB family. The C-erbB-2 is a proto-oncogene located on human chromosome 17, band 21. It has structural  similarities to the EGFR (Epidermal Growth Factor Receptor). Immunohistochemical staining of the oncoprotein is related to gene amplification. Different studies have demonstrated immunohistochemical detectable levels in more than 20% of adenocarcinomas of different locations, including ovarian, gastro-intestinal tract and breast. In the case of breast cancer, the overexpression has been shown to be associated with poor prognosis. This antibody is recommended to determine the expression levels of the oncoprotein C-erb B-2 in breast adenocarcinomas and other locations.

Composition: anti-cerbB2 rabbit monoclonal antibody obtained from supernatant culture and prediluted in a tris buffered solution pH 7.4 containing 0.375mM sodium azide solution as bacteriostatic and bactericidal. Intended use : Immunohistochemistry (IHC) on paraffin embedded tissues. Not tested on frozen tissues or Western-Blotting

Immunogen: Recombinant protein encoding extracellular domain of human c-erbB2

Species reactivity: In vitro diagnostics in humans. Not tested in other species

.(Name: VIPAntibody(Clone H-6

Description and applications: Glucagon is a pancreatic hormone that functions as an antagonist to Insulin, stimulating the conversion of glycogen to glucose and increasing blood sugar levels. Glucagonlike peptide-1 (GLP-1), glucagon-like peptide-2 (GLP- 2), VIP (vasoactive intestinal peptide) and PACAP (pituitary adenylate cyclase activating polypeptide) are members of the glucagon family of hormones. GLP-1 functions as a transmitter in the central nervous system, inhibiting feeding and drinking behavior, whereas GLP-2 is a stimulator of intestinal epithelial growth. VIP causes vasodilation resulting in the lowering of blood pressure. PACAP is abundant in the hypothalamus and has been shown to increasethe synthesis of several hormones, including growth hormone.

Composition: Anti-human VIP mouse monoclonal antibody purified from serum and prepared in PBS with < 0.1% sodium azide and 0.1% gelatin.

Immunogen: Antibody raised against amino acids 1-95 of vasoactive intestinal peptide VIP of human origin.

.(Name: Vimentin Antibody (Clone SP20

Description and applications: Vimentin is the main intermediate filament protein in mesenchymal cells and is therefore of value in the differential diagnosis of undifferentiated neoplasms. In mesenchymal tumors, vimentin expression is practically the rule, though, it must not forget that there are epithelial tumors that coexpressed Vimentin and cytokeratins, such as thyroid gland carcinomas, pleomorphic adenomas salivary of the salivary glands and some renal carcinomas. Furthermore, by the universal staining the anti-vimentin antibody in the blood vessels and lymphoid tissue, they are considered the best internal control to assess the results of immunostaining.

Composition: anti-human Vimentin, rabbit monoclonal antibody purified from culture supernatant, prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Immunogen: Recombinant protein encoding human vimentin.

.(Name: Villin-1 Antibody (Clone CWWB1

Description and applications:Villin is a tissue-specific protein with a molecular massAnti-villin monoclonal antibody (CWWB1 clone) against the human antigen has a sensitivity of almost 100% and high specificity; thus, immunostaining is fully reproducible using standard amplification and development procedures performed as part of automated immunostaining systems. Villin is expressed in the brush border of enterocytes and kidney proximal tubule cells. In addition to allowing immunohistochemical identification of villin in normal human tissues, this antibody is a highly specific marker of gastrointestinal tumours and pancreatic cancer. Although with less sensitivity, renal, hepatic and pulmonary neoplasms, especially carcinoid tumours, neuroendocrine tumours and signet-ring cell carcinomas or carcinomas with microlumens filled with microvilli, as well as some breast and ovarian tumours, are also stained. of 95.5 kDa. Of all proteins belonging to the gelsolin family, it is the only one capable of assembling and disassembling actin filaments through three specific binding domains, two of them Ca2+-dependent. Villin is essential for the assembling of the cell brush border citoskeleton and the organization of the actin filament bundle forming the core of microvilli. In addition, it has been proposed that this protein, due to its dependence on cell activation via phosphatidylinositol, is essential for the control of cell polarity during embryonic development of tissues containing it, as well as to facilitate epithelial plasticity in response to cell injury and immune system cell elements migration through the intestinal barrier.

Composition:Anti-human Villin-1 mouse monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Immunogen:uman villin protein.

.(Name: Tyrosinase Antibody (Clone T311

Description and applications:Melanin synthesis by melanocytes comprises a cascade of reactions involving a family of enzymes. One of the key enzymes is tyrosinase. L-tyrosine is the initial substrate for melanin synthesis and its conversion to dopaquinone is catalysed by tyrosinase, the expression of which is therefore a marker of melanocytes and melanoma. Tyrosinase deficiency is associated with various forms of albinism, specially oculocutaneous albinism. This antibody is useful for the identification of melanocytic tumours and it may be used in combination with other markers such as Melan A. Both markers are expressed in 80-100% of melanoma cases.

Composition: Anti-human Tyrosinase mouse monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Immunogen: Recombinant protein corresponding to the tyrosinase molecule.

.(Name: Thyroid Transcription Factor 1 (TTF-1)(clone SPT24

Description and aplications:This antibody recognizes a protein identified as thyroid transcription factor 1 (TTF-1), a member of the Nkx2 family homeodomain transcription factors. This antibody was designed against a short recombinant peptide and shows great sensitivity and provides a stronger and less cytoplasmic unspecific staining or cross-reaction with tissues other than thyroid and lung. The clone SPT24 identifies the presence of TTF-1 in thyroid follicular epithelium, as well as type II pneumocytes and Clara cells of the lung and their corresponding tumors. It can also be observed in certain brain reactive glial cells and some cases of colon adenocarcinomas. It shows no nuclear staining in a variety of normal tissues and no cytoplasmic reactivity was described in hepatocarcinomas.

Composition: anti- TTF1 mouse monoclonal antibody obtained from supernatant culture and prediluted in a tris buffered solution pH 7.4 containing 0.375mM sodium azide solution as bacteriostatic and bactericidal. The quantity of the active antibody was not determined.

.Immunogen: recombinant protein molecule corresponding to a fragment of 123 AA of the N-terminal region of human TTF-1