دسته: آنتی بادیهای ایمونوهیستوشیمی
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آنتی بادیهای ایمونوهیستوشیمیآنتی بادی Inhibin Alpha کلون INHA/4265 برند Patho Sage
Specification:
It recognizes a 47kDa protein, which is identified as alpha sub-unit of Inhibin. It is a gonadal protein that preferentially suppresses the secretion of pituitary follicle-stimulating hormone (FSH). Inhibin comprises two subunits, Inhibin A and Inhibin B. Each subunit consists of the same subunit, covalently linked to 1 of 2 distinct subunits, – or -. Originally isolated from ovarian follicular fluid and characterized as a disulphide-linked dimeric glycoprotein, inhibin belongs to the transforming growth factor (TFG) superfamily. Antibodies against Inhibin are useful in making a differentiation between adrenal cortical tumors and renal cell carcinoma. Sex cord stromal tumors of the ovary as well as trophoblastic tumors also demonstrate cytoplasmic positivity. Inhibin antibody is also used to make the differential diagnosis of intra-uterine vs. ectopic pregnancy in endometrial curetting.
Immunogen:
A synthetic peptide (around aa 73-96) of α-subunit of human inhibin A protein (exact sequence is proprietary)
Presentation:
Bioreactor Concentrate with 0.05% Azide
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آنتی بادیهای ایمونوهیستوشیمیآنتی بادی CD31 (PECAM-1) کلون JC/70A برند Patho Sage
Specification:
CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. It is widely used as an endothelial cell marker. CD31 Ab reacts with normal, benign, and malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis, and a high level of CD31 expression may imply a rapidly growing tumor and potentially a predictor of tumor recurrence.
Immunogen:
Membrane preparation of a spleen from a patient with hairy cell leukemia
Presentation:
Bioreactor Concentrate with 0.05% Azide
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آنتی بادیهای ایمونوهیستوشیمیآنتی بادی Cytokeratin 8/18 کلون K.8 + DC10 برند Patho Sage
Specification:
Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratin’s and exists in combination with cytokeratin 18 (CK18). This MAb cocktail recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells; there is very little keratin 18 in the normal epidermis so only Paget cells are stained. Immunohistochemical staining with this MAb is indistinguishable from that obtained with monoclonal antibody 5D3.
Immunogen:
Keratin preparation from a human carcinoma (K8.8); PMC-42 human breast carcinoma cells (DC10)
Presentation:
Protein A/G purified antibody from Bioreactor Concentrate with 0.05% BSA and 0.05% Azide.
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آنتی بادیهای ایمونوهیستوشیمیآنتی بادی Cytokeratin PAN کلون LU5 برند Patho Sage
Specification:
Monoclonal antibody Lu-5 reacts with an epitope which is common to all cytokeratins respectively with a cytokeratin associated protein. The antigen recognized by anti-cytokeratin pan is present in all types of epithelia. It recognizes most subtypes of cytokeratins in cryofixed and paraffin sections and is ideally suited as a first order pan-epithelial marker. The epitope recognized by Lu-5 is a formalin-resistant marker of great value in tumor diagnosis, located on the surface of cytokeratin filaments. It has been preserved during vertebrate evolution and can be shown in all species from amphibia to man. The epitope is present in most cytokeratin polypeptides of both the acidic (type I) and basic (type II) subfamily but does not occur in other cytoskeletal proteins. The epithelial specificity and the broad tissue and species cross reactivity provide an excellent probe for the differential diagnosis of epithelial versus mesenchymal tumors, large cell lymphomas and neural tumors.
Immunogen:
Lung tumor cell lines A549 and A2182
Presentation:
Concentrated cell culture supernatant in phosphate buffered saline pH 7.2 (PBS), and 0.05% sodium azide as a preservative
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آنتی بادیهای ایمونوهیستوشیمیآنتی بادی MELAN A (MART1) کلون M2-9E3 برند Patho Sage
Specification:
This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MAb labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive marker for angiomyolipoma’s. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.
Immunogen:
Recombinant hMART-1 protein
Presentation:
Bioreactor Concentrate with 0.05% Azide
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آنتی بادیهای ایمونوهیستوشیمیآنتی بادی CDX2 کلون MonoPoly/6271R برند Patho Sage
Specification:
The specificity of this monoclonal antibody to its intended target was validated by HuProt TM Array, containing more than 19.000 full-length human proteins. The intestine-specific transcription factors CDX1 and CDX2 are important for directing intestinal development, differentiation, proliferation and maintenance of the intestinal phenotype. CDX2 protein expression has been seen in GI carcinomas. Anti-CDX2 has been useful to establish GI origin of metastatic adenocarcinoma and carcinoids and is especially useful in distinguish metastatic colorectal adenocarcinoma from lung adenocarcinoma. However, mucinous carcinomas of the ovary also expressed CDX2 protein. It limits the usefulness of this marker in the distinction of metastatic colorectal adenocarcinoma from mucinous of the ovary.
Immunogen:
Recombinant fragment and/of synthetic peptides of human CDX2 protein
Presentation:
Bioreactor Concentrate with 0.05% Azide
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آنتی بادیهای ایمونوهیستوشیمیآنتی بادی CD43 کلون MT1 برند Patho Sage
Specification:
CD43 antigen is expressed on all T cells, NK cells, myeloid cells and monocytes as well as on CD5 positive mature B cells. It is also present on all immature hematopoietic cells in the bone marrow. The antigen is deficient in patients with Wiskott-Aldrich syndrome. A soluble form of CD43 is present in human serum.CD43, clone MT1 antibodies are used to identify B cell lines and myeloma cells. It may be used in the diagnosis of chronic lymphocytic leukemias (CLL), as an alternative to CD5. The antigen detected by MT1 is the only marker expressed on neoplasms of the very early precursor cells. In immunohistochemistry it reacts with T cells, macrophages, myeloid cells and B cells (weak). It is used for the typing of lymphomas in paraffin sections. CD43 may function as an adhesion molecule via interaction with CD54 although this has not been definitely established. It may also inhibit leucocyte interactions with other cells. The antigen is involved in the activation of T-cells, B cells, NK cells and monocytes. The membrane proximal portion of the cytoplasmic domain mediates an association with the cytoskeleton.
Presentation:
Antibodies are supplied in 0.01M sodium phosphate, 0.15 M NaCl, pH 7.3, 0.2% BSA, 0.09% sodium azide
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آنتی بادیهای ایمونوهیستوشیمیآنتی بادی Helicobacter Pylori کلون MX014 برند Patho Sage
Specification:
Anti-H.pylori antibody reacts with H.pylori typically found on the surface of pyloric and stomach mucosa. Studies have shown that H.pylori plays an important role in the ethology of chronic gastritis and the development of peptic ulcer disease.
Presentation:
Tissue culture supernatant containing 15mM sodium azide.
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آنتی بادیهای ایمونوهیستوشیمیآنتی بادی CD19 کلون MX016 برند Patho Sage
Specification:
CD19 is expressed only on B-cells and follicular dendritic cells. It is a specific and sensitive marker of B-cells widely expressed from early pre-B stages, normal B-cells and normal plasma cells (staining is weaker than normal B-cells and a subpopulation may lack expression). It is considered a positive regulator of both intrinsic and stimulus-dependent pathways in B-lymphocytes. CD19 is useful in identification of B-cell lineage of majority of B-cell neoplasms but appears to be less useful in subclassifying of B-cell neoplasms in histological material. It appears to be potentially useful additional marker of follicular dendritic cell tumors.
Presentation:
Liquid tissue culture supernatant containing 15mM sodium azide
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آنتی بادیهای ایمونوهیستوشیمیآنتی بادی Calretinin کلون MX027 برند Patho Sage
Specification:
Calretinin is a 29 kDa calcium-binding protein, member of the family of so-called EF-hand proteins, to which also the S-100 proteins belong. Calretinin is abundantly expressed in neurons. Outside the nervous system, calretinin is found in mesothelial cells, steroid producing cells (adrenal cortical cells, testicular Leydig cells, ovarian theca internal cells), testicular Sertoli cells, rete testis, ovarian surface epithelium, some neuroendocrine cells, breast glands, eccrine sweat glands, hair follicular cells, thymic epithelial cells, endometrial stromal cells and fat cells. In calretinin positive cells, the protein is generally found in both cytoplasm and nuclei.
Presentation:
Tissue culture supernatant containing 15mM sodium azide
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آنتی بادیهای ایمونوهیستوشیمیآنتی بادی CD31 (PECAM-1) کلون MX032 برند Patho Sage
Specification:
CD31 is a transmembrane glycoprotein, 130-140 kDa, also designated platelet-endothelium cell adhesion molecule = PECAM-1, belonging to the immunoglobulin super family. CD31 is ligand for CD38 and plays a role in thrombosis and angiogenesis. CD31 is strongly expressed in endothelial cells and weakly expressed in megakaryocytes, platelets, occasional plasma cells, lymphocytes (esp. marginal zone B-cells, peripheral T-cells) and neutrophils.
Presentation:
Tissue culture supernatant containing 15mM sodium azide.
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آنتی بادیهای ایمونوهیستوشیمیآنتی بادی Inhibin Alpha کلون MX098 برند Patho Sage
Specification:
Inhibin is a heterodimeric glycoprotein with a molecular weight of 32.000 composed of an alpha-subunit along with a β Asubunit, inhibin Alpha or a βB-subunit, inhibin-β. Inhibin alpha and inhibin-β are synthesized by the gonads and regulate the secretion by the pituitary of follicle-stimulating hormone. Inhibin-alpha increased in the late follicular phase. And the pattern of secretion of the inhibin A suggest inhibin a may indicate follicle maturity.
Presentation:
Supernatant contain 15mM Sodium Azide
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آنتی بادیهای ایمونوهیستوشیمی
آنتی بادی CD43 کلون SPN/3388 برند Patho Sage
Specification:
It recognizes a cell surface glycoprotein of 95/115/135kDa (depending upon the extent of glycosylation), identified as CD43. 70-90% if T-cell lymphomas and from 22-37% of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be malignant lymphoma, especially a low grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.
Immunogen:
Recombinant full-length human SPN protein
Presentation:
Purified antibody from bioreactor concentrates by protein A/G. Prepared in 10mM PBS with 0.05% BSA and 0.05% Azide.
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آنتی بادیهای ایمونوهیستوشیمی
آنتی بادی WT1 (Wilms Tumor) کلون WT49 برند Patho Sage
Specification:
The WT1 gene, identified as a tumor suppressor gene located at 11p13, is involved in the development of Wilms’ tumor. The WT1 gene encodes a transcription factor with four DNA-binding zinc fingers at the C terminus. In vitro studies showed that WT1 suppresses or activates a number of genes, including those for PDGF-A chain, EGF receptor, CSF-1, IGF-II, IGF-I receptor, RAR-α, c-myc, bcl-2 and WT1 itself. Immunohistochemically, WT1 is detected in the nucleus of tumor cells of Wilms’ tumor and mesothelioma; therefore, WT1 has traditionally been used as a diagnostic marker for these tumors. Recent reports showed that other types of cancers, such as ovarian serous cancers and rhabdomyosarcomas also express WT1.
Presentation:
Tissue culture supernatant containing 15mM sodium azide
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آنتی بادیهای ایمونوهیستوشیمیآنتی بادی PR (Progesterone Receptor) کلون ZR4 برند Patho Sage
Specification:
The progesterone receptor (PgR) is an estrogen regulated protein. It has been proposed that expression of PgR determination indicates a responsive estrogen receptor (ER) pathway, and therefore, may predict likely response to endocrine therapy in human breast cancer. A number of studies have shown that PgR determination provides supplementary information to ER, both in predicting response to endocrine therapy and estimating survival. PgR has proved superior to ER as a prognostic indicator in some studies.
Immunogen:
Recombinant protein encoding human progesterone receptor 412-526 aa
Presentation:
Tissue culture supernatant with 0.2% BSA and 15mM sodium azide
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آنتی بادیهای ایمونوهیستوشیمی
آنتی بادی Borrelia Burgdorferi کلون Polyclonal برند Patho Sage
Specification:
This antibody reacts with Borrelia burgdorferi and is known to cross react with Treponema pallidum, Borrelia hermsii, and Borrelia parkerii.
Immunogen:
Whole cell preparation from B. burgdorferi
Presentation:
Polyclonal purified IgG fraction, 4-5mg/ml in 10mM PBS, pH 7.2 with Sodium Azide.
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آنتی بادیهای ایمونوهیستوشیمیآنتی بادی CEA (Carcinoembryonic Antigen) کلون Polyclonal برند Patho Sage
Specification:
Anti-CEA specifies a group of proteins in the Carcinoembryonic Antigen (CEA) family of proteins which are present in the epithelia of various types and tumors (both benign and malignant) derived from such epithelia. Such tissues are represented by the epithelia of colon, bronchus, alveoli, breast, pancreas, biliary tract, superficial layer and parietal layers of the stomach. Predominately biliary canaliculi are labeled in the liver and this factor is useful in the diagnosis of hepatocellular carcinoma. Anti-CEA has been quite useful in differentiating adenocarcinoma of the lung vs. mesothelioma.
Presentation:
Anti-CEA is a rabbit polyclonal antibody, diluted in tris buffered saline, pH 7.3-7.7, with protein base, and preserved with sodium azide
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آنتی بادیهای ایمونوهیستوشیمی
آنتی بادی Prolactin کلون Polyclonal برند Patho Sage
Specification:
Anti-Prolactin is a useful marker in classification of pituitary tumors and the study of pituitary disease. It reacts with prolactin-producing cells. Such prolactin-producing cells can also be found in prostate epithelium.
Presentation:
Rabbit polyclonal antibody purified from rabbit anti-sera diluted in tris buffered saline, pH 7.3-7.7, with protein base, and preserved with sodium azide
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آنتی بادیهای ایمونوهیستوشیمی
آنتی بادی S100 Protein کلون Polyclonal برند Patho Sage
Specification:
S-100 protein has been found in normal melanocytes, Langerhans cells, histiocytes, chondrocytes, lipocytes, skeletal and cardiac muscle, Schwann cells, epithelial and myoepithelial cells of the breast, salivary and sweat glands, as well as in glial cells. Neoplasms derived from these cells also express S-100 protein, albeit non-uniformly. A large number of well differentiated tumors of the salivary gland, adipose and cartilaginous tissue, and Schwann cell derived tumors express S-100 protein. Almost all malignant melanomas and cases of histiocytosis x are positive for S-100 protein. Despite the fact that S-100 protein is an ubiquitous substance, its demonstration is of great value in the identification of several neoplasms, particularly melanomas. The antibody reacts with human and cow S-100 protein A and B. In the brain it labels glial and ependymal cells. Melanocytes and Langerhans cells of the skin also stain positive.
Immunogen:
Affinity purified S-100 protein isolated from cow brain
Presentation:
Purified immunoglobulin fraction of rabbit antiserum against S100 containing sodium azide as a preservative
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آنتی بادیهای ایمونوهیستوشیمی
آنتی بادی SOX10 کلون 1074 برند Patho Sage
Specification:
Recognizes a protein of -55kDa, identified as SOX I 0. This MAb is highly specific and does not cross-react with other members of the SOX-family. SOX genes comprise a family of genes that are related to the mammalian sex-determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequencespecific DNA binding activity. SOX-10 is a sensitive marker of melanoma, including conventional, spindled,and desmoplastic subtypes. It is expressedby metastatic melanomas and nodal capsular nevusin sentinel lymph nodes, but notby other lymph node componentssuch asdendritic cells, which usually express S I 00protein. Commonly used melanoma markers, such as anti-HMB-45andanti-Melan-A, are poorly expressed in desmoplasticmelanomas while SOX-10 is moderately to strongly expressed in desmoplastic melanomas. SOX- 10 is considered as a very reliable marker for recognizing residual desmoplastic melanomas. In normal tissues, it is expressed in Schwann cells, melanocytes, and myoepithelial cells of salivary, bronchial and mammary glands. SOX-I 0 expression is also observed in mast cells.
Immunogen:
Recombinant fragment (155 Amino residues between aa 100-300) of human SOX10 protein
Presentation:
Bioreactor Concentrate with 0.05% Azide