Name: Flow Cytometry Antibody CD13-PE, Clone WM15
- Antibody CD13-PE is a monoclonal antibody (mAb) labelled with R-phycoerythrin (PE) designed for flow cytometry (FC) use as a direct immunofluorescence reagent in the identification and enumeration of CD13 antigen-expressing cells. CD13 can be considered a panmyeloid antigen, as it is expressed on the surface of most myeloid origin cells.
SUMMARY AND EXPLANATION
FC is a powerful tool in analytical and quantitative characterization of cells which provides rapid and multiparametric analysis of heterogeneous cell populations on a cell-by-cell basis. Flow cytometry is performed on cell suspension after incubating it with fluorescentlabelled antibodies directed against specific cellular proteins. Positive cells relative fluorescence intensity indicates the amount of antibody bonded to specific cell sites providing information about antigen expression. CYT-13PE3 mAb recognizes CD13 antigen, a single-chain integral membrane glycoprotein present on most cells of myeloid origin including neutrophils, eosinophils, basophils and monocytes from normal peripheral blood .CD13 antigen is also found on the surface of granulocyte-macrophage progenitor cells in normal bone marrow. This molecule is expressed in the majority of subjects with acute myeloid leukemias (AML) and can be aberrantly expressed in cases of acute lymphoblastic leukemia (ALL). This antibody can therefore be used in the characterization of hematologic neoplasia.
The purified monoclonal CD13 antibody conjugated with R-phycoerythrin (PE) is supplied in phosphate-buffered saline (PBS) containing 0.1% sodium azide.
- Clone: WM15.
- Isotype: IgG1.
- Amount per 1 ml vial: 100 tests (10 µl mAb per determination).
- Reagent is considered non-sterile