Name: Flow Cytometry Antibody CD21-APC-C750™, Clone Bly4
- Antibody CD21-APC-C750TM is a monoclonal antibody (mAb) labelled with the tandem allophycocyanine-C750 (APC-C750TM) designed for Flow Cytometry (FC) use as a direct immunofluorescence reagent in the identification and enumeration of CD21 antigen-expressing cells.
SUMMARY AND EXPLANATION
FC is a powerful tool in analytical and quantitative characterization of cells which provides rapid and multiparametric analysis of heterogeneous cell populations on a cell-by-cell basis. FC is performed on cell suspension after incubating it with fluorescent-labelled antibodies directed against specific cellular proteins. Positive cells relative fluorescence intensity indicates the amount of antibody bonded to specific cell sites providing information about antigen expression.
CYT-21AC750 mAb specifically binds to CD21, a 145 kDa glycosylated type I integral membrane protein, also known as the Complement receptor type 2 (CR2) or Epstein-Barr virus (EBV) receptor. It is expressed on mature B cells, follicular dendritic cells, some epithelial cells and very weakly on T cells. CD21 plays a role in B-cell activation and proliferation. It may also play a role in modulating the function of T cells in the immune response to infections by lymphotropic viruses. CD21 antibodies have been shown to bind CD23 and IFN-a. CD21 is part of a multimeric complex on B cells which includes CD19 and CD81. Anti CD21 MAb is applied in the immunophenotyping of leukemias and lymphomas. APC-C750TM is a tandem dye with a maximum emission peak at 779 nm, which grants bright signal, low unspecific noise and high photostability. When excited by light from a red laser, the APC fluorochrome can transfer energy to C750 molecule, which then emits at a longer wavelength. It is recommended to use a 780/60 nm longpass filter along with a red sensitive detector to use in conjunction antibodies conjugated with APC and APC-C750TM.
The purified monoclonal CD21 antibody conjugated with the tandem allophycocyanine-C750 (APC-C750TM) is supplied in phosphate-buffered saline (PBS) containing 0.09% (m/v) sodium azide.
- Clone: B-ly4.
- Isotype: IgG1.
- Amount per 0,15 ml vial: 50 tests (3 µl mAb per determination).
- Reagent is considered non-sterile.