Name: Flow Cytometry Antibody CD22-FITC, Clone B-ly8
- Antibody CD22-FITC is a monoclonal antibody (mAb) labelled with Fluorescein Isothiocyanate (FITC) designed for flow cytometry use as a direct immunofluorescence reagent in the identification and enumeration of CD22 antigen-expressing cells.
SUMMARY AND EXPLANATION
Flow Cytometry (FC) is a powerful tool in analytical and quantitative characterization of cells which provides rapid and multiparametric analysis of heterogeneous cell populations on a cell-by-cell basis. Flow cytometry is performed on cell suspension after incubating it with fluorescent-labelled antibodies directed against specific cellular proteins. Positive cells relative fluorescence intensity indicates the amount of antibody bonded to specific cell sites providing information about antigen expression. Human lymphocytes may be classified in three main populations according to their biological function and their cell surface antigen expression (T lymphocytes, B lymphocytes and natural killer (NK) cells. CD22 antigen (also named SIGLEC-2) is a type I transmembrane glycoprotein present on the surface of mature B and activated B cells (but not on plasma cells), and in the cytoplasm of pro-B and pre-B cells. CD22 has been reported to participate in B-cell activation via surface Ig and also as an adhesion molecule. CD22 is used in flow cytometry for the immunophenotyping of B cell lineage leukemias and lymphomas. Aberrant CD22 expression is useful for differentiating neoplastic from benign B- cells. CD22 is overexpressed in hairy cell leukemia and splenic marginal zone lymphoma, whereas is under expressed or shows an asynchronous expression in other B-chronic lymphoproliferative disease groups, including chronic lymphocytic leukemia, prolymphocytic leukemia, mantle-cell lymphoma, follicular lymphoma and lymphoplasmacytic lymphoma.
The purified monoclonal CD22 Antibody conjugated with Fluorescein Isothiocyanate (FITC) is supplied in phosphate-buffered saline (PBS) containing 0.1% sodium azide.
- Clone: B-ly8.
- Isotype: IgG1.
- Amount per 1 ml vial: 200 tests (5 µl mAb per determination).
- Reagent is considered non-sterile.