Name: Flow Cytometry Antibody CD22-PE, Clone MEM-01
- Antibody CD22-PE is a monoclonal antibody (mAb) labelled with R-phycoerythrin (PE) designed for flow cytometry (FC) use as a direct immunofluorescence reagent in the identification and enumeration of CD22 antigen-expressing cells. This reagent must be used by FC qualified personal.
SUMMARY AND EXPLANATION
Human lymphocytes may be classified in three main populations according to their biological function and their cell surface antigen expression (T lymphocytes, B lymphocytes and natural killer (NK) cells). CD22 antigen (also named SIGLEC-2) is a type I transmembrane glycoprotein present on the surface of mature B and activated B cells (but not on plasma cells), and in the cytoplasm of pro-B and pre-B cells. CD22 has been reported to participate in B-cell activation via surface Ig and also as an adhesion molecule. CD22 is used in flow cytometry for the immunophenotyping.
REAGENT COMPOSITION
The purified monoclonal CD22 Antibody conjugated with R-phycoerythrin (PE) is supplied in phosphate-buffered saline (PBS) containing 0.09% sodium azide.
- Clone: MEM-01.
- Isotype: IgG1.
- Amount per 1 mL vial: 200 tests (5 mL mAb per determination).
- Reagent is considered non-sterile.
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