Name: Flow Cytometry Antibody CD33-FITC, Clone WM53
- CD33-FITC is a monoclonal antibody (mAb) labelled with fluorescein isothiocyanate (FITC) designed for use as a direct immunofluorescence reagent in the identification and enumeration of cells which express the CD33 antigen by flow cytometry (FC). Anti-CD33 is considered essential for the initial evaluation of acute myeloid leukemia (AML) together with a panel of other antibodies. This reagent must be used by FC qualified personal.
SUMMARY AND EXPLANATION
The CYT-33F mAb reacts with a member of the siglec family of 67kDa expressed by peripheral blood monocytes, neutrophils, granulocyte precursors, myeloid progenitor cells and cells of myeloid leukemias. Analysis of the expression of antigen CD33 is useful for the identification and the characterization of hematologic disorders since CD33 has been found on the cell surface of leukemic blasts from the majority of cases of AML. Leukemic lymphoblasts do not show any expression of this antigen.
Purified monoclonal CD33 antibody conjugated with fluorescein isothiocyanate (FITC), supplied in phosphate buffered saline with 0,09% (m/v) sodium azide.
- Clone: WM53
- Isotype: IgG1
- Purification: Affinity chromatography
- Amount per 1 mL vial: 200 tests (5 µL mAb to 106 cells)
- Reagent is not considered sterile.