Name: Flow Cytometry Antibody CD33-PE, Clone MW53
- Antibody CD33-PE is a monoclonal antibody (mAb) labelled with R-phycoerythrin (PE) designed for flow cytometry (FC) use as a direct immunofluorescence reagent in the identification and enumeration of CD33 antigen-expressing cells.
SUMMARY AND EXPLANATION
FC is a powerful tool in analytical and quantitative characterization of cells which provides rapid and multiparametric analysis of heterogeneous cell populations on a cell-by-cell basis. Flow cytometry is performed on cell suspension after incubating it with fluorescentlabelled antibodies directed against specific cellular proteins. Positive cells relative fluorescence intensity indicates the amount of antibody bonded to specific cell sites providing information about antigen expression. CD33 antigen is a 67 kD type I transmembrane glycoprotein restricted to myelomonocytic lineage. CD33 is expressed on monocytes and macrophages, granulocytes, myeloid progenitors (not the earliest precursors), and myeloid dendritic cells. It is absent on normal platelets, lymphocytes, erythrocytes, and hematopoietic stem cells. CD33 expression is brighter in monocytes and dimmer in mature granulocytes (expression decreases with maturation and differentiation). CD33 antibody is used in flow cytometry for identifying and immunophenotyping myeloid leukemias. It is a high valuable antigen to distinguish acute myeloid leukemias from their lymphoblastic and erythroblastic counterparts. It is also a key marker to diagnose myelodysplastic syndromes by detecting dysplasia in the different myeloid compartments.
The purified monoclonal CD33 Antibody conjugated with R-phycoerythrin (PE) is supplied in phosphate-buffered saline (PBS) containing 0.1% sodium azide.
- Clone: WM53.
- Isotype: IgG1.
- Amount per 1 ml vial: 200 tests (5 µl mAb per determination).
- Reagent is considered non-sterile.