Name: Flow Cytometry Antibody CD4-PE-Cyanine5, Clone RPA-T4
- CD4 is a monoclonal antibody (mAb) labelled with different fluorochromes (see table), designed for use as a direct immunofluorescence reagent in the identification and enumeration of cells which express the CD4 antigen by flow cytometry (FC). This reagent must be used by flow cytometry qualified personal.
SUMMARY AND EXPLANATION
Human lymphocytes may be classified in three main populations according to their biological function and their cell surface antigen expression: T lymphocytes, B lymphocytes and natural killer cells (NK). T lymphocytes (CD3+), the precursors of which originate in the bone marrow and then migrate and mature in the thymus, can be subdivided as well in functionally different populations. The most clearly defined of these are helper/inducer T cells (CD3+CD4+) and suppressor/cytotoxic T cells (CD3+CD8+). T cells produce no antibodies and are the mediators of cell immunity. CD4 mAb recognizes the CD4 antigen present in helper/inducer T cells (CD3+CD4+) and can therefore be used in the characterization studies for immunophenotyping of lymphocytes. These studies are widely applied for monitoring of the immunologic status of post-transplant patients and in the characterization and follow-up of immunodeficiencies, autoimmune diseases, leukemia etc. Individuals with HIV typically exhibit a steady decrease of helper/inducer T lymphocyte counts as the infection progresses. The CD4 antigen is also detected on some monocytes.
Purified monoclonal CD4 antibody conjugated with different fluorochromes (see table above) is supplied in phosphatebuffered saline (PBS) containing 1% (m/v) BSA and 0,09% (m/v) sodium azide.
- Purification: Affinity chromatography
- Amount per vial: 50, 100 or 200 tests (3 or 5 µl mAb to 106 cells) (see table).
- Reagents are not considered sterile