Name: Flow Cytometry Antibody CD5-PerCP-Cyanine5.5, Clone L17F12
- Antibody CD5-PerCP-Cyanine5.5 is a monoclonal antibody (mAb) labelled with Peridin chlorophyll protein-Cyanine 5.5 (PerCPCyanine5.5), designed for use as a direct immunofluorescence reagent in the identification and enumeration of cells which express the CD5 antigen by Flow Cytometry (FC). CD5 is a useful marker for the identification of certain lymphoprolipherative syndromes when is used within an extended immunophenotyping panel. This reagent must be used by flow cytometry qualified personal.
SUMMARY AND EXPLANATION
The CYT-5C2 mAb recognizes a 67 kDa antigen expressed by a majority of thymocytes and mature T cells and a subset of B cells. CD5 is expressed at low density on thymocytes and at high density on all mature T lymphocytes. The majority of T cell malignancies expresses CD5, and almost 85% of T cell acute lymphoblastic leukemias are CD5 positive. Further, CD5 is expressed at low density on a small subset of mature B lymphocytes, which is expanded in several autoimmune disorders as well as in some B-cell derived lymphoproliferative disorders such as chronic lymphocytic leukemia (CLL) and centrocytic leukemia.
Purified monoclonal CD5 antibody conjugated with conjugated with PerCP-Cyanine5.5, supplied in phosphate-buffered saline (PBS) containing 1% (m/v) BSA and 0.09% (m/v) sodium azide.
- Clone: L17F12
- Isotype: Mouse / IgG2a
- Purification: Affinity chromatography
- Amount per vial: 50 tests (3 ml mAb to 106 cells) Reagents are not considered sterile.