Name: Flow Cytometry Antibody CD5-FITC, Clone MCD5
- Antibody CD5-FITC is a monoclonal antibody (mAb) labelled with fluorescein isothiocyanate (FITC) designed for use as a direct immunofluorescence reagent in the identification and enumeration of cells which express the CD5 antigen by flow cytometry (FC). CD5 is a useful marker for the identification of certain lymphoprolipherative syndromes when is used within an extended immunophenotyping panel. This reagent must be used by flow cytometry qualified personal.
SUMMARY AND EXPLANATION
The CYT-5F4 mAb recognizes a 67 kDa antigen expressed by a majority of thymocytes and mature T cells and a subset of B cells. CD5 is expressed at low density on thymocytes and at high density on all mature T lymphocytes. The majority of T cell malignancies expresses CD5, and almost 85% of T cell acute lymphoblastic leukemias are CD5 positive. Further, CD5 is expressed at low density on a small subset of mature B lymphocytes, which is expanded in several autoimmune disorders as well as in some B-cell derived lymphoproliferative disorders such as chronic lymphocytic leukemia (CLL) and centrocytic leukemia.
Purified monoclonal CD5 antibody conjugated with fluorescein isothiocyanate (FITC), supplied in phosphate buffered saline with 0,09% sodium azide.
- Clone: MCD5
- Isotype: IgG2b
- Purification: Affinity chromatography
- Amount per 1 ml vial: 200 tests (5 ml mAb to 106 cells)
- Reagents are not considered sterile