Name: Flow Cytometry Antibody CD66b-FITC, Clone 80H3
- Antibody CD66b-FITC is a monoclonal antibody (mAb) labelled with fluorescein isothiocyanate (FITC) designed for flow cytometry (FC) use as a direct immunofluorescence reagent in the identification and enumeration of CD66b antigen-expressing cells.
SUMMARY AND EXPLANATION
FC is a powerful tool in analytical and quantitative characterization of cells which provides rapid and multiparametric analysis of heterogeneous cell populations on a cell-by-cell basis. Flow cytometry is performed on cell suspension after incubating it with fluorescentlabelled antibodies directed against specific cellular proteins. Positive cells relative fluorescence intensity indicates the amount of antibody bonded to specific cell sites providing information about antigen expression. Haematopoietic cells give rise to all blood cell types from myeloid (e.g. monocytes, neutrophils, basophils and erythrocytes) and lymphoid (e.g. T, B and NK cells) lineages. Several markers have been routinely used to distinguish both lineages; an example is the CD66b which is used to identify mature granulocytes (neutrophils and eosinophils). CD66b is a GPI-linked protein member of the carcinoembryonic antigen (CEA)-like glycoprotein that was first described in colon cancer cells and is not expressed in normal peripheral B cells, T cells, monocytes and platelets. CD66b has an increase in its expression in granulocytes activated with soluble stimulators (calcium ionophore, phorbol myristate acetate, nFMLP). CD66 proteins are involved in several cellular processes (e.g. cell adhesion and activation). Eosinophils CD66b expression increases in patients with Helminth infection. Also, leukocytes of Chronic Myeloid Leukaemia (CML) present CD66b expression.
The purified monoclonal CD66b Antibody conjugated with fluorescein isothiocyanate (FITC) is supplied in phosphate-buffered saline (PBS) containing 0.1% sodium azide.
- Clone: 80H3.
- Isotype: IgG1.
- Amount per 1 ml vial: 200 tests (5 µl mAb per determination).
- Reagent is considered non-sterile.