Name: Flow Cytometry Antibody FMC7-FITC, Clone FMC7
- Antibody FMC7-FITC is a monoclonal antibody (mAb) labelled with fluorescein isothiocyanate (FITC) designed for flow cytometry (FC) use as a direct immunofluorescence reagent in the identification and enumeration of FMC7 antigen-expressing cells.
SUMMARY AND EXPLANATION
FC is a powerful tool in analytical and quantitative characterization of cells which provides rapid and multiparametric analysis of heterogeneous cell populations on a cell-by-cell basis. Flow cytometry is performed on cell suspension after incubating it with fluorescentlabelled antibodies directed against specific cellular proteins. Positive cells relative fluorescence intensity indicates the amount of antibody bonded to specific cell sites providing information about antigen expression. The FMC-7 antibody detects a glycoprotein of 105kD found on circulating B-lymphocytes. Studies on normal lymphocytes, leukaemic cells and cell lines indicate that FMC-7 is a marker for a limited segment of the B cell maturation pathway. This glycoprotein is expressed in varying degrees on normal circulating B-cells, chronic B-cell leukaemia (B-CLL), prolymphocytic leukaemia (PLL) and other B cell neoplasias. It stains peripheral blood B lymphocytes and tonsil B lymphocytes. No reaction with granulocytes, monocytes, platelets, erythrocytes, T lymphocytes or null cells. The FMC-7 antibody reacts with HRIK and Raji cell lines.
The purified monoclonal FMC7 antibody conjugated with fluorescein isothiocyanate (FITC) is supplied in phosphate-buffered saline (PBS) containing 0.1% sodium azide.
- Clone: FMC7
- Isotype: IgM
- Amount per 1 ml vial: 200 tests (5 µl mAb per determination)
- Reagent is considered non-sterile.