Name: Flow Cytometry Antibody HLA-B27-FITC, Clone HLA–ABC–m3
- HLA-B27-FITC is a monoclonal antibody (mAb) labelled with fluorescein isothiocyanate (FITC) designed for flow cytometry (FC) use as a direct immunofluorescence reagent in the identification and enumeration of HLA-B27 antigen-expressing cells.
SUMMARY AND EXPLANATION
FC is a powerful tool in analytical and quantitative characterization of cells which provides rapid and multiparametric analysis of heterogeneous cell populations on a cell-by-cell basis. Flow cytometry is performed on cell suspension after incubating it with fluorescentlabelled antibodies directed against specific cellular proteins. Positive cells relative fluorescence intensity indicates the amount of antibody bonded to specific cell sites providing information about antigen expression. Human Leukocyte Antigen (HLA) B27 is a class I surface antigen encoded by the B locus in the major histocompatibility complex (MHC) on chromosome 6 and presents antigenic peptides to T cells. HLA-B27 and HLAB7 antigens are expressed in 7% and 22% of individuals of Caucasian origin. This mixture of antibodies permits the characterization of HLA-B27 specificity in the HLA class I allotype in patients suffering from inflammatory disorders affecting the sacroiliac and intervertebral joints. This finding aids in the diagnosis of ankylosing spondylitis 90% of sufferers of which express the HLA-B27 antigen, versus 7% in the normal population
The purified monoclonal HLA-B27 Antibody conjugated with fluorescein isothiocyanate (FITC) is supplied in phosphate-buffered saline (PBS) containing 0.1% sodium azide.
- Clone: HLA–ABC–m3.
- Isotype: IgG2a
- Amount per 1 ml vial: 200 tests (5 µl mAb per determination).
- Reagent is considered non-sterile.