Name: Flow Cytometry Antibody Lambda-FITC, Clone Polyclonal
- Antibody Lambda-FITC in a goat polyclonal antibody (pAb) labelled with fluorescein isothiocyanate (FITC) and designed for use as a direct immunofluorescence reagent in the identification and enumeration of cells that express human Lambda immunoglobulin Light Chains by flow cytometry. Antibodies to Lambda Light Chains are useful for the identification of clonal excess in B-cell lymphoproliferative disorders together with a panel of other antibodies. Anti-Lambda Light Chains reacts with free Lambda chains as well as Lambda chains in intact immunoglobulin molecules. This reagent must be used by flow cytometry qualified personal.
SUMMARY AND EXPLANATION
Human lymphocytes may be classified in three main populations according to their biological function and their cell surface antigen expression: T lymphocytes, B lymphocytes and Natural Killer cells (NK). B lymphocytes are the producers of antibodies and mediate humoral immunity particularly effective against toxins, whole bacteria, and free viruses. Most B cells, with the exception of pre-B progenitors, pre B-cells, and mature plasma cells, express immunoglobulin on their surface. Each cell expresses only one light chain type (Kappa or Lambda). In normal peripheral blood and lymph nodes, there is a mixture of Kappa positive and Lambda positive cells, with two-thirds of the cells expressing Kappa and one-third expressing Lambda. Since lymphoid neoplasms are usually clonal expansions of a single cell, malignant cells uniformly express the same Light Chain isotype. Neoplastic B cell lymphoprolipherative disorders can frequently be suspected based on the demonstration of a marker predominance of cells expressing a single Light Chain type. The Lambda immunoglobulin Light Chain count is generally expressed as a percentage of B cells present in the sample that can be determined by flow cytometry based on its positive expression of CD19. Since every flow cytometer has different operating characteristics each laboratory must determine its optimal operating procedure and the combination of markers used to identify the target cell population.
Purified polyclonal antibody Anti-human Lambda Light Chains, Goat F(ab’)2, conjugated with fluorescein isothiocyanate (FITC), supplied in phosphate buffered saline with 0,09% (m/v) sodium azide.
- Purification: Affinity chromatography
- Amount per 0,5 mL vial: 100 tests (5 µL pAb to 106 cells).
- Reagents are not considered sterile.