Name: Flow Cytometry Antibody NG2-PE, Clone 7.1
- Antibody NG2-PE is a monoclonal antibody (mAb) labelled with R-phycoerythrin (PE) designed for flow cytometry (FC) use as a direct immunofluorescence reagent in the identification and enumeration of NG2 antigen-expressing cells.
SUMMARY AND EXPLANATION
FC is a powerful tool in analytical and quantitative characterization of cells which provides rapid and multiparametric analysis of heterogeneous cell populations on a cell-by-cell basis. Flow cytometry is performed on cell suspension after incubating it with fluorescentlabelled antibodies directed against specific cellular proteins. Positive cells relative fluorescence intensity indicates the amount of antibody bonded to specific cell sites providing information about antigen expression. NG2, is an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma and other cells and plays a role in angiogenesis-dependent processes. NG2 has been associated with chromosomal aberrations in approximately 1-10 % of AML (Acute myeloid leukemia) patients. NG2 has been selected by EuroFlow consortium in its AML and BCP-ALL antibody panels.
The purified monoclonal NG2 antibody conjugated with R-phycoerythrin (PE) is supplied in phosphate-buffered saline (PBS) containing 0.1% sodium azide.
- Clone: 7.1
- Isotype: IgG1.
- Amount per vial: 100 tests (10 µl mAb per determination).
- Reagent is considered non-sterile.