Name: Flow Cytometry Antibody CD2-FITC, Clone RPA-2.10
- Antibody CD2-FITC is a monoclonal antibody (mAb) labelled with fluorescein isothiocyanate (FITC) designed for use as a direct immunofluorescence reagent in the identification and enumeration of cells which express the CD2 antigen by flow cytometry (FC). CD2 can be considered a pan-T cell antigen, as it is expressed on the surface of all normal Tlymphocytes. This reagent must be used by flow cytometry qualified personal.
SUMMARY AND EXPLANATION
The CYT-2F7 mAb recognizes the 50kDa lymphocyte surface antigen expressed on all peripheral blood T lymphocytes, the majority of thymocytes and malignant cells of T cell origin, including T ALL cells. CD2 is a useful marker in the assessment of lymphoid malignancies as it is expressed in the majority of precursor and posthymic lymphomas and leukemias. In some neoplastic T-cell populations e.g. in peripheral T cell lymphomas, CD2 may be aberrantly deleted.
REAGENT COMPOSITION
Purified monoclonal CD2 antibody conjugated with fluorescein isothiocyanate (FITC), supplied in phosphate buffered saline with 0,09% sodium azide.
- Clone: RPA-2.10
- Isotype: IgG1
- Purification: Affinity chromatography
- Amount per 1 mL vial: 200 tests (5 µL mAb to 106 cells)
- Reagents are not considered sterile.
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