آنتی‌بادی Prostatic Acid Phosphatase-کلون PASE/4LJ

Available Volume: 3ml / 7ml / 12ml / Concentrated

Composition: Anti-human Prostatic Acid Phosphatase mouse monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide

Intended use: Immunohistochemistry (IHC) on paraffin embedded tissues. Not tested on frozen tissues or Western-Blotting

Clone: PASE/4LJ

Ig isotype: mouse IgG1

IHC positive control: Prostate

Visualization: Cytoplasmic

توضیحات و استفاده

Description and applications: Anti-PSAP reacts with prostatic acid phosphatase in the glandular epithelium of normal and hyperplastic prostate, carcinoma of the prostate and metastatic cells of prostatic carcinoma. This marker may be helpful in pinpointing the site of origin in cases of metastatic carcinoma of the prostate, and is considered a more sensitive marker than PSA. However, it also offers less specificity. Nevertheless, PSAP complements PSA in the right clinical context.

Storage and stability: up to 18 months; stored at 2-8ºC. Do not freeze.

IHC recommended procedure: 

4μm thick section should be taken on charged slides; dry overnight at 60ºC

Deparaffinise, rehydrate and HIER (heat induced epitope retrieval) – boil tissue in the Pt Module using Master Diagnóstica EDTA buffer pH83 for 20 min at 95ºC. Upon completion rinse with 3-5 changes of distilled or deionised water followed by cooling at RT for 20 min

Endogenous peroxidase block – Blocking for 10 minutes at room temperature using peroxidase solution (ref. MAD-021540Q-125)

Primary antibody: incubate for 10 minutes [The antibody dilution (when concentrated) and protocol may vary depending on the specimen
preparation and specific application. Optimal conditions should be determined by the individual laboratory]

For detection use Master Polymer Plus Detection System (HRP) (DAB included; ref. MAD-000237QK)

Counterstaining with haematoxylin and final mounting of the slide

دیدگاهها

هیچ دیدگاهی برای این محصول نوشته نشده است.

اولین نفری باشید که دیدگاهی را ارسال می کنید برای “آنتی‌بادی Prostatic Acid Phosphatase-کلون PASE/4LJ”

نشانی ایمیل شما منتشر نخواهد شد.

عنوان

Go to Top