Name: Flow Cytometry AntibodyCD3-APC-C750™, Clone UCHT-1

• Antibody CD3 reagent is a monoclonal antibody (mAb) conjugated with different fluorochromes (see table) and designed for use as a direct immunofluorescence reagent in the identification and enumeration of cells which express the CD3 antigen by flow cytometry. This reagent must be used by flow cytometry qualified personnel.

SUMMARY AND EXPLANATION

The anti-CD recognizes the CD3 antigen present in T cells and can therefore be used in the characterization studies for immunophenotyping of lymphocytes. These studies are widely applied for monitoring of the immunologic status of posttransplant patients and in the characterization and follow-up of immunodeficiencies, autoimmune diseases, leukemia etc. The T lymphocyte (CD3+) count is generally expressed as a percentage of the total amount of lymphocytes or leucocytes present in the sample which can itself be determined by flow cytometry based on its typical pattern of FSC/SSC (size/granularity or complexity). Because each flow cytometer has different operating characteristics each laboratory must determine its optimal operating procedure.

 REAGENT COMPOSITION

Purified monoclonal CD3 antibody conjugated with different fluorochromes (see table above) is supplied in phosphatebuffered saline (PBS) containing 1% (m/v) BSA and 0.09% (m/v) sodium azide.

• Clone: UCHT-1
• Isotypes: Mouse / IgG1
• Purification: Affinity chromatography
• Amount per vial: 50 or 200 tests (3 or 5 µl mAb to 106 cells).
• Reagents are not considered sterile.