آنتی بادی مونوکلونال فلوسایتومتری ™ CD20-Pacific Blue، کلون 2H7

Name: Flow Cytometry Antibody CD20-Pacific Blue™, Clone 2H7

• Antibody Anti-human CD20 reagent is a monoclonal antibody (mAb) conjugated with different fluorochromes (see table) and designed for use as a direct immunofluorescence reagent in the identification and enumeration of cells which express the CD20 antigen by Flow Cytometry (FC). This reagent must be used by FC qualified personal.

SUMMARY AND EXPLANATION

Human lymphocytes may be classified in three main populations according to their biological function and their cell surface antigen expression (T lymphocytes, B lymphocytes and natural killer (NK) cells). B lymphocytes are the producers of antibodies, they mediate humoral immunity particularly effective against toxins, whole bacteria, and free viruses. CD20 can be considered a pan-B cell antigen, as it is expressed on the surface of all mature B-lymphocytes but not in secreting plasma cells. Anti-human CD20 reagent recognizes CD20 antigen present on B cells and can therefore be used in lymphocytes immunophenotyping. Antibodies to CD20 are widely applied in the characterization and follow-up of acute and chronic lymphoproliferative disorders. Pacific BlueTM is excited with the violet laser (405nm) and emits at 550nm. This fluorochrome provides maximum resolution and narrow emission peaks, which results in little spectral overlap and minimal compensation requirements. In multicolor panel it is recommended the use of this fluorochrome combined with Orange Cytognos 515, which is also excited with the violet laser and emits at 515nm.

REAGENT COMPOSITION

Purified anti-human CD20 mAb conjugated with different fluorochromes (see table above) is supplied in phosphatebuffered saline (PBS) containing 0,09% (m/v) sodium azide

• Amount per vial: 100 or 200 tests (5 µl mAb per determination) (see table above).
• Reagent is considered non-sterile.