Name: ALK (Anaplastic Lymphoma Kinase) / p80 antibody
Description and aplications: This antibody recognizes the hybrid p80NPM/ALK chimeric protein od 80kDa, a product of the t (2; 5) (p23; q35) translocation between the anaplastic lymphoma kinase gene and the nucleophosmin gene which is specifically expressed in 40% of Ki-1 lymphoma (anaplastic large cell lymphoma). In addition to the anaplastic large cell lymphoma, the antibody can be used for the identification of lung adenocarcinomas harbouring rearrangements of the ALK gene and which have shown dramatic improvement Other neoplasm and reactive conditions like some diffuse large B cell lymphomas (DLBCL), neuroblastomas, rare anaplastic carcinomas of the thyroid, kidney, oesophagus, colon and breast, various sarcomas (rare rhabdomyosarcomas, lipogenic tumors, Ewing/PNET sarcomas and leyomiosarcomas) or the inflammatory myofibroblastic tumour might also express the protein not necessary related with the specific translocation.
Composition: anti- ALK mouse monoclonal antibody obtained from supernatant culture and prediluted in a Tris buffered solution pH 7.4 containing 0.375mM sodium azide solution as bacteriostatic and bactericidal. The quantity of the active antibody was not determined.
Immunogen: Recombinant protein corresponding to a region which spans the tyrosine kinase catalytic domain and part of the C-terminus of the NPM-ALK transcript (419-520aa)