Specificity, Interference and Limitation: EDTA buffer is used in methods for heat induced antigen retrieval (HIER). When used on material fixed in buffered formalin and embedded in paraffin, certain antibodies require antigen unmasking for retrieving the immunoreactivity that had been masked by the fixing process.
Storage: Store until the expiry date of the product at room temperature, away from any sources of intense heat or cold.
Application: The HIER has improved immunohistochemical staining in conventional surgical pathology as it improves the antigen unmasking and the staining intensity. The use of these techniques has its best advantages in case of nuclear antigens that presents a marked increase in signal intensity. No previous dewaxing step is necessary, as this product has been design as an all-in-one solution that is suitable for simultaneous dewaxing and heat induced antigen retrieval.
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