پلی کلونال

Name: Carcino embryonic Antigen (CEAp) Antibody Polyclonal

Description and applications:This antibody has a high affinity for CEA and shows no detectable reactivity to nonspecific cross-reacting antigen (NCA), biliary glycoprotein (BGP) and human polymorphonuclear leucocytes. Ab-3 shows no reaction with a variety of normal tissues .CEA is not found in benign glands, stroma, or malignant prostatic cells. Antibody to CEA is useful in detecting early foci of gastric carcinoma and in distinguishing pulmonary adenocarcinomas (60-70% are CEA+) from pleural mesotheliomas (rarely or weakly CEA+).

Composition: anti-human CEA rabbit polyclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Immunogen: Human CEA isolated from hepatic metastasis of colon adenocarcinoma.

Name: Caspase 3 Polyclonal Antibody

Description and aplications: Caspase 3 is ubiquitously expressed and like other caspases is synthesized as an inactive (32kDa) proenzyme. Upon activation, Caspase 3 is cleaved at Asp28-Ser29 and Asp175-Ser176 thereby generating two subunits of 17kDa and 12kDa, respectively. Recent studies have implicated that Caspase 3 is associated with the induction of apoptosis. Activation of Caspase 3 occurs in response to variety of apoptotic inducers including Fas mediated apoptosis. This antibody detects both active and inactive forms of Caspase-3 protein. The antibody is useful for studying the phenomenon of cell death by apoptosis as a pathogenic mechanism of numerous pathologies: nerve damage, neurodegenerative diseases, cardiovascular diseases, autoimmune diseases, AIDS and malignancies.

Composition: anti-human Caspase 3 rabbit polyclonal antibody purified from rabbit anti-serum by Protein A chromatography. Prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide

Immunogen: Recombinant full length human.

Name: BOB-1 (SP92)

Description and applications: The BOB.1, OBF.1, OCA-B (now called BOB.1 / OBF.1) is a 35 kD protein encoded by a gene located on chromosome 11q23.1. BOB.1 from “B cell binding Octamer protein 1 “is a specific coactivator of transcription factors Oct-1 and Oct-2 of B lymphocytes. BOB.1 mission is to increase transcriptional activity of such proteins. Interaction with transcription factor OCT2 octamer ATGCAAAT nucleotides is critical and decisive for the expression of immunoglobulin genes. It has been recently demonstrated that alteration in immunoglobulin transcription is correlated with the downregulation of the transcription factors Oct-2 and BOB.1 / OBF.1 on B lymphocytes. This antibody recognizes BOB.1 of human, murine and rat origin. In normal lymphoid tissue, BOB.1 is expressed mainly in the nuclei of germinal center B lymphocytes, with lower intensity in the follicular mantle and dispersed form in B lymphocytes localized in the interfolicular areas of the lymphoid tissue; for this reason it is useful in identifying B cell lymphomas. Variable intensity has been demonstrated in Hodgkin disease, classic type without statistical relationship with its different subtypes. The expression of specific activator proteins of B lymphocytes like “octamer-binding transcription factor 2” [Oct-2] and BOB.1 with pan-B lymphocyte markers (CD20 or CD79a ) are useful for the differential diagnosis of diffuse large B-cell lymphoma, nodular lymphocyte-predominant Hodgkin lymphoma (OCT-2 + / BOB.1 + / PAN-B +) with anaplastic lymphoma B (OCT-2 / BOB. 1- / PAN-B +). The antibody shows no cross-react with Oct-1 or Oct-2.

Composition: anti-human BOB-1 rabbit monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide

Intended use: Immunohistochemistry (IHC) on paraffin embedded tissues. Not tested on frozen tissues or Western-Blotting

Name: Helicobacter Pylori Polyclonal Antibody

Description and applications: This antibody detects the Campylobacter jejuni, although some of the Campylobacter jejuni flagellar antigens are shared by other subspecies of Campylobacters like Helicobacter pylori.

Composition: anti-Helicobacter Pylori rabbit polyclonal antibody obtained from supernatant culture and prediluted in a tris buffered solution pH 7.4 containing 0.375mM sodium azide solution as bacteriostatic and bactericidal.

Immunogen: Purified Helicobacter Pylori

Name: Placental Lactogen (hPL) Polyclonal Antibody

Description and applications: Human placental lactogen (hPL) is known to originate in the syncytiotrophoblasts. Ultrastructural localization indicates that placental lactogen is stored in small morphologically distinctcytoplasmic granules within these cells and has adifferent secretory mechanism from other hormonal peptides such as human chorionic gonadotropin.

The level of expression of placental lactogen through pregnancy increases gradually to term. Immunostaining for hPL is useful for the differential diagnosis of choriocarcinoma where it is expressed in most cases, placental site trophoblastic tumour and the trophoblastic pseudotumoral lesions such as placental site nodule and exaggerated placental site reaction.

Composition: anti-human Placental Lactogen rabbit polyclonal antibody purified from rabbit anti-serum. Prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide

Immunogen: Purified human placental lactogen

Name: Treponema Pallidum Antibody (Polyclonal)

Description and applications: The antitreponema antibody included in this test consists of a purified fraction of rabbit IgG and is highly specific for the determination of spirochetes. With the increasing incidence of human immunodeficiency virus (HIV) infection and immunosuppressive therapy in various pathologies, the incidence of syphilis is growing in many parts of the world. Lesions that develop during secondary syphilis are difficult to diagnose clinically and they may even be accompanied by a negative serology. Likewise, the histopathological changes observed in these cases do not always meet the typical diagnostic criteria. Classically, spirochetes location was performed using silver impregnations such as Steiner and/or Warthin- Starry. Nevertheless, the identification of Treponema pallidum can now be successfully performed using immunohistochemical techniques on formalin-fixedparaffin-embedded tissues. In this sense, several studies have shown that these procedures, due to their greater sensitivity and specificity, offer significant advantages over traditional silver impregnations. This antibody displays cross-reactivity with Borrelia burgdorferi.

Composition: anti-human Treponema Pallidum rabbit polyclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Immunogen: Treponema pallidum.

Name: CXCL13 Antibody (Polyclonal)

Description and applications: CXCL13, also known as B lymphocyte chemoattractant (BLC), is a CXC chemokine that is constitutively expressed in secondary lymphoid organs. BCA1 cDNA encodes a protein of 109 amino acid residues with a leader sequence of 22 residues. Mature human BCA1 shares 64% amino acid sequence similarity with the mouse protein and 23-34% amino acid sequence identity with other known CXC chemokines. Recombinant or chemically synthesized BCA1 is a potent chemoattractant for B lymphocytes but not T lymphocytes, monocytes or neutrophils. BLR1, a G protein coupled receptor originally isolated from Burkitt’s lymphoma cells, has now been shown to be the specific receptor for BCA1. Among cells of the hematopoietic lineages, the expression of BLR1, now designated CXCR5, is restricted to B lymphocytes and a subpopulation of T helper memory cells. Mice lacking BLR1 have been shown to lack inguinal lymph nodes. These mice were also found to have impaired development of Peyer’s patches and defective formation of primary follicles and germinal centers in the spleen as a result of the inability of B lymphocytes to migrate into B cell areas. The CXCL13 expression in angioimmunoblastic T-cell lymphoma contribute in their diagnostic.

Composition: anti-human CXCL13 goat monoclonal antibody antigen affinity-purified. Prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Immunogen: E. coli derived recombinant human CXCL13/BCA1 Val23Arg94

Name: Chorionic Gonadotrophin (hCG) Polyclonal Antibody

Description and applications: hCG is a protein secreted in large quantities by normal trophoblasts; the antibody detects cells and tumours of trophoblastic origin such as Choriocarcinoma. Large Cell Carcinoma and Adenocarcinoma of Lung demonstrate hCG positivity in 90% and 60% of cases respectively. 20% of Squamous Cell Lung Carcinomas are positive for hCG. hCG expression by non-trophoblastic tumours may indicate aggressive behavior since it has been observed that hCG may play a role in the host response to a given tumour.

Composition: anti-hCG rabbit polyclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide

Name: Adipophilin Antibody (Polyclonal)

Description and applications: Sebaceous carcinoma is a relatively uncommon cutaneous malignancy and mimics other malignant neoplasms, such as basal and squamous cell carcinomas, and benign processes, such as chalazions and blepharitis, sometimes resulting in delayed diagnosis and suboptimal treatment. Adipophilin is present in milk fat globule membranes and on the surface of lipid droplets in various normal cell types. Recently, it has been reported that adipophilin was expressed in sebaceous adenomas with a specific pattern: membranous with strong uptake at the periphery of intracytoplasmic lipid vacuoles. Sebaceous carcinomas are also labeled with a similar pattern. Additionally, in cases of poorly differentiated sebaceous carcinoma, adipophilin highlights the sebocytes with a strong membranous labeling of intracytoplasmic lipid droplets. Moreover, xanthelasmas, xanthogranulomas, xanthomas, metastatic renal cell carcinomas were also weakly-to- moderately positive for adipophilin in one study. Expression of adipophilin with a membranous pattern of staining was not seen in any of the other clear cell lesions of the skin, including basal and squamous cell

carcinomas, trichilemmomas, clear cell hidradenomas,or balloon cell nevi. Interestingly, a nonspecific granular uptake of anti-adipophilin was seen in adjacent macrophages, keratohyalin granules of epithelial squamous cells, and some tumor cells.Therefore, this anti-adipophilin is suitable for immunostaining formalin-fixed, paraffin-embedded tissue and is helpful in the identification of intracytoplasmic lipids, as seen in sebaceous lesions. Acinary cell carcinomas of the breast, some liposarcomas, or carcinomas of the colon, lung, pancreas or prostate and strangely the majority of Burkitt lymphomas might also be positive.

Composition:Anti-human Adipophilin rabbit polyclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Name: Poly (ADP-Ribose) Polymerase 1 Antibody

Description and applications: PARP (EC 2.4.2.30) is a nuclear protein with a molecular mass of 116 kDa and two zinc finger motifs which binds to DNA and specifically detects DNA nicks and breaks produced by different genotoxic agents. PARP catalises the ADP-ribosylation of proteins using NAD+ as a substrate. PARP activation is a consequence of ischemic injury and leads to intracellular depletion of NAD+, which can only be replaced by ATP consumption. Ischemia/Reperfusion (I/R) injury results in substantial DNA damage and cells need to consume large amounts of ATP to sustain reparative poly-ADP-ribosylation. Proteolysis of PARP to its stable 85-kDa fragment is an early marker of programmed cell death (apoptosis), mediated by caspase 3 or CPP32. Cleavage occurs between Adp216 and Gly217, a site in PARP conserved across species. This antibody reacts with human, mouse and rat PARP. PARP-1 determination may be useful for detecting cells undergoing DNA replication or repair. In ischemia/reperfusion situations, it may serve as an early marker of cell death. Studies on chemical carcinogenesis have involved PARP on its pathogenesis. Recently, PARP has been considered a therapeutic target in some types of malignancies such as breast carcinomas with BRCA1/2 mutation.

Composition: anti-human Poly (ADP-Ribose) Polymerase 1 rabbit polyclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide

Intended use: Immunohistochemistry (IHC) on paraffin embedded tissues. Not tested on frozen tissues or Western-Blotting

Immunogen: Synthetic peptide derived from the Nterminal region of human protein PARP.

Name: IgD Antibody (Polyclonal)

Description and applications: Anti-IgD antibody reacts with surface immunoglobulin IgD delta chains. This antibody is useful when identifying leukemias, plasmacytomas, and B-cell lineage derived lymphomas (in particular Marginal Zone Lymphoma). Cytoplasmic staining is easily identified on paraffin tissue. Surface immunoglobulin is dificult to demonstrate on paraffin sections but can be demonstrated on frozen sections.

Composition: anti-human IgD rabbit polyclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide

Name: IgM Antibody (Polyclonal)

 Description and applications: Anti-IgM antibody reacts with surface immunoglobulin IgM mu chains. IgM is one of the predominant surface immunoglobulins on B-lymphocytes. This antibody is useful when identifying lymphomas, plasmacytomas, and B-cell lineage derived Hodgkin’s lymphomas. Due to the restricted expression of heavy and light chains in these diseases, demonstration of B-cell lymphomas is possible with clonal gene rearrangement studies.

Composition: Anti-human IgM rabbit polyclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide