Name: Poly (ADP-Ribose) Polymerase 1 Antibody
Description and applications: PARP (EC 220.127.116.11) is a nuclear protein with a molecular mass of 116 kDa and two zinc finger motifs which binds to DNA and specifically detects DNA nicks and breaks produced by different genotoxic agents. PARP catalises the ADP-ribosylation of proteins using NAD+ as a substrate. PARP activation is a consequence of ischemic injury and leads to intracellular depletion of NAD+, which can only be replaced by ATP consumption. Ischemia/Reperfusion (I/R) injury results in substantial DNA damage and cells need to consume large amounts of ATP to sustain reparative poly-ADP-ribosylation. Proteolysis of PARP to its stable 85-kDa fragment is an early marker of programmed cell death (apoptosis), mediated by caspase 3 or CPP32. Cleavage occurs between Adp216 and Gly217, a site in PARP conserved across species. This antibody reacts with human, mouse and rat PARP. PARP-1 determination may be useful for detecting cells undergoing DNA replication or repair. In ischemia/reperfusion situations, it may serve as an early marker of cell death. Studies on chemical carcinogenesis have involved PARP on its pathogenesis. Recently, PARP has been considered a therapeutic target in some types of malignancies such as breast carcinomas with BRCA1/2 mutation.
Composition: anti-human Poly (ADP-Ribose) Polymerase 1 rabbit polyclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide
Intended use: Immunohistochemistry (IHC) on paraffin embedded tissues. Not tested on frozen tissues or Western-Blotting
Immunogen: Synthetic peptide derived from the Nterminal region of human protein PARP.