Name: Rabbit anti-human C4d Polyclonal Antibody
Description and applications: This antibody recognizes the C4d fraction of complement. C4d fraction of complement represents the alpha-2 (42 kDa) portion of the C4 fraction of complemnto. Among the different complement proteins, the opsonins C3 and C4 have a protector thioester radical. This molecule allows the C4 (and C3) complement fraction to form covalent bonds when activated with the target molecule, thereby generating the C4b (C3b) molecule. When the proteolytic cleavage of C4b occurs, C4d fragment remains covalently attached to the target structure, while the C4c fragment is free.
Consecutively, C4d is a stable split product remnant of classical complement activation and an established marker of antibody-mediated acute renal allograft rejection. Due to its proclivity for endothelium, this component can be detected in peritubular capillaries in both chronic renal allograft rejection as well as hyperacute rejection, acute vascular rejection, acute cellular rejection, and borderline rejection. It has been shown to be a significant predictor of transplantkidney graft survival and is an aid in treating acute rejection.
Composition: anti-human C4d rabbit polyclonal antibody purified from ascites. Prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide
Intended use: Immunohistochemistry (IHC) on paraffin embedded tissues. Not tested on frozen tissues or Western-Blotting