Name: CD23 Monoclonal Antibody clone 1B12
Description and applications: The role of IgE immunoglobulins in the initiation and development of acute allergic processes is well known. These processes are generally initiated through its interaction with high affinity receptors for Fc portion (FceRI) located mainly on the surface of mast cells and basophil granulocytes where it regulates exocytosis phenomena and subsequent degranulation of mediators of hypersensitivity reactions type I. Furthermore, the immunoglobulin IgE also binds specifically to an integral membrane glycoprotein of 45- 55 kDa type 2 (321 aa) that does not belong to the immunoglobulin superfamily but to the selectins and are included under the name of low affinity receptor of IgE (FceRIIa+b / CD23). CD23 is expressed in a wide range of cell types, including B lymphocytes, eosinophils, granulocytes, platelets, T and NK cells, dendritic reticulum cells and Langerhans cells. CD23 expression has been detected in neoplastic lymphoid cells of chronic lymphocytic leukemia, in a variable percentage of follicular lymphomas and in a small proportion of large B-cell lymphomas. Furthermore and although the CD23 molecule is produced by B cells, is adsorbed in a large amount on follicular dendritic reticulum cells thus is also used for the identification of its neoplasms (dendritic reticulum cell sarcoma).
Composition: anti-CD23 mouse monoclonal antibody obtained from supernatant culture and prediluted in a tris buffered solution pH 7.4 containing 0.375mM sodium azide solution as bacteriostatic and bactericidal.
Immunogen: Recombinant external domain of CD23 protein.