Name: Cytokeratin HMW Antibody clone 34beta
Description and applications: The proteins generically known intermediate filaments measuring between 7 and 22 nm diameter (ie with a size between -5 to 7 nm like actin and 22 to 25 nm like tubulin), are part, together with the above mentioned, of the vertebrates cytoskeleton. This superfamily comprises six subfamilies of molecules with different tissue expression. Cytokeratins constitute two homology groups (I and II) and in humans are encoded by more than 49 different genes on chromosomes 17 (I) and 12 (II). The nomenclature coined in 1982 by Moll and Franke assigns ranges of 1 to 8 for type II cytokeratins (neutralor basic) and between 9 and 21 for type I (acidic). Normally, keratins are assembled in heterodimers I / II and are co-expressed in pairs specific to each tissue. This antibody recognizes keratins of 68, 58, 56.5 and 50 kD obtained from the skin stratum corneum. By immunohistochemistry, the antibody reacts with squamous epithelium, ductal epithelia and other similar structure. This antibody is used confirm carius types of tumors like breast, pancreatic, bile duct, salivary gland, prostate and transitional cell carcinoma.
Composition: anti-HMW-CK mouse monoclonal antibody obtained from supernatant culture and prediluted in a tris buffered solution pH 7.4 containing 0.375mM sodium azide solution as bacteriostatic and bactericidal.
Immunogen: Solubilized keratin extracted from human stratum corneum.