پروب فیش IGH-MAF Fusion/Translocation FISH Probe

INTRODUCTION: MAF gene encode in humanas the Transcription factor Maf also known as proto-oncogene c-Maf or V-maf musculoaponeurotic fibrosarcoma oncogene homolog. t(14;16)(q32;q23) represents 14q32/IGH rearrangement and belongs to the group of IGH/MAF translocations (rearrangements of the genes from the MAF oncogene family MAF, MAFA and MAFB with the IGH locus). As the other two IGH/MAF translocations t(8;14)q24;q32) andt(14;20)(q13;32) is described only in plasma cell neoplasms (PCN). t(14;16)(q32;q23) resulted in the juxtaposition of the oncogene MAF ( located at 16q23) to the strong enhancer of the IGH gene (located at 14q32) causing its up regulation in the plasma cells. This anomaly is found in both multiple myeloma (MM) and its precursor monoclonal gammapathy of undetermined significance (MGUS) respectively in 5% and 1-5% of the cases with 14q32 rearrangements.
INTENDED USE: The IGH-MAF Fusion/Translocation FISH probe kit is designed to detect rearrangements involving regions of the human IGH locus, located on chromosome band 14q32.33, and of the human MAF gene on 16q23.2.

PROBE DESCRIPTION: The IGH Probe of the IGH-MAF Fusion/Translocation FISH Probe Kit labeled with the fluorochrome CytoGreen covers the 5’ and the center sequences of the IGH locus, and it also covers the 3’ (end) part and the neighboring downstream region. The MAF Probe of the IGH-MAF Fusion/Translocation FISH Probe Kit labeled with the fluorochrome CytoOrange covers the chromosomal region that includes the entire MAF gene.