آنتی­ بادی Thrombomodulin (EP175)

Name: Thrombomodulin  Antibody (Clone EP175)

Description and applications: Thrombomodulin (TM) is a transmembrane glycoprotein of 75 kDa molecular mass with six repeated domains homologous to the epidermal growth factor and a terminal domain homologous to the lectin-like proteins. Thrombomodulin actives anticoagulant proteins derived from vitamin K synthesized in the liver (proteins C and S) which bind to thrombin in order to prevent thrombus formation in the endothelial surface of the vessel. TM expression is upregulated by agents that increase cAMP and down through the interleukin I, tumor necrosis factor (TNF) and some endotoxins. The TM 1009, bindsto domains 4 and 6 of the epidermal growth factor, the smallest region which binds to thrombin. This binding may interfere with the binding between thrombin and thrombomodulin. This antibody produced immunostaining in a variety of normal cells: endothelial lining blood vessels and lymphatics cells, mesothelial cells, normal urothelium, some pulmonary alveolar macrophages, coating meningeal cells, synoviocytes, syncytiotrophoblasts, megakaryocytes and platelets. Thrombomodulin is also found in some subtype cell of pancreatic islets and in peripheral nerves. It is diagnostically useful in neoplastic cells of vascular tumors, urothelial carcinomas, meningiomas, adenomatoid tumor, choriocarcinoma and pleural tumors. TM can be used for differential diagnosis between mesothelioma (positive) and lung adenocarcinoma (consistently negative). Weak expression of TM was demonstrated in two thirds of the cases examined, while a strong TM staining was revealed in one third of colon cancers.

Composition: anti-Thrombomodulin rabbit monoclonal antibody obtained from supernatant culture and prediluted in a tris buffered solution pH 7.4 containing
0.375mM sodium azide solution as bacteriostatic and bactericidal.

Immunogen: A synthetic peptide corresponding to residues at the C-terminus of human thrombomodulin protein.