دسته: آنتی بادیهای ایمونوهیستوشیمی

Specification:

Recognizes a sialo glycoprotein of 27-32kDa, identified as CD99, or MIC2 gene product, or E2 antigen. MIC2 gene is located in the pseudo-autosomal region of the human X and Y chromosome. MIC2 gene encodes two distinct proteins, which are produced by alternative splicing of the CD99 gene transcript and are identified as bands of 30 and 32kDa (p30/32). Although its function is not fully understood, CD99 is implicated in various cellular processes including homotypic aggregation of T cells, upregulation of T cell receptor and MHS molecules, apoptosis of immature thymocytes and leukocyte diapedesis. CD99 is expressed on the cell membrane of some lymphocytes, cortical thymocytes, and granulosa cells of the ovary. Most pancreatic islet cells, Sertoli cells of the testis, and some endothelial cells express this antigen. Mature granulocytes express very little or no CD99. MIC2 is strongly expressed on Ewing’s sarcoma cells and primitive peripheral neuroectodermal tumors.

Immunogen:

Human acute lymphocytic leukemia T-cells

Presentation:

Bioreactor Concentrate with 0.05% Azide

Specification:

The catenin’s alpha, beta, and gamma are proteins which bind to the highly conserved, intracellular cytoplasmic tail of Ecadherin. Together, the catenin/cadherin complexes play an important role mediating cellular adhesion. Catenin, a 102 kDaprotein, was initially described as an E-cadherin-associated protein and has been shown to associate with other members of the cadherin family N-cadherin and P-cadherin. The 92 kDa catenin associates with the cytoplasmic portion  of E-cadherin which is necessary for the function of E-cadherin as an adhesion molecule. Catenin has also been found in complexes with the tumor suppressor protein APC. Catenin, also known as plakoglobin, is an 82 kDa protein that binds with catenin and Ncadherin. A related protein, p120, exhibits sequence homology with the catenins at four discreet domains. p120 not only serves as a substrate for Src but is also found in E-cadherin complexes containing catenins. p120 (15D2): sc-23872. Immunoperoxidase staining of formalin fixed, paraffin-embedded human breast tissue showing membrane and cytoplasmic localization.

Presentation:

Each vial contains 200 µg IgG in 1.0 ml of PBS with < 0.1% sodium azide 1 and 0.1% gelatin

Specification:

The LIM-only (LMO) proteins, LMO1 and LMO2, are nuclear factors that are characterized by a conserved LIM domain. The LIM domain consists of a cysteine-rich zinc-binding motif that is present in a variety of transcription factors, including the LIM homeobox (LHX) proteins expressed in the central nervous system and involved in cell differentiation. LMO1 and LMO2 are expressed in the adult CNS in a cell type-specific manner, where they are differentially regulated by neuronal activity and are involved in regulating the cellular differentiated phenotype of neurons. LMO2 lacks a specific DNA-binding homeobox domain but rather assembles into transcriptional regulatory complexes to mediate gene expression by interacting with the widely expressed nuclear LIM interactor (NLI). NLI, known also as CLIM-1, and the related protein CLIM2 facilitate the formation of heteromeric LIM complexes and also enhance the nuclear retention of LIM proteins. LMO2 and the related protein LMO4 are expressed in thymic precursor cells. LMO4 is also expressed in mature T cells, cranial neural crest cells, somite, dorsal limb bud mesenchyme, motor neurons, and Schwann cell progenitors.

Immunogen:

Recombinant LMO2 of human origin

Presentation:

Each vial contains 200 µg IgG1 in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Specification:

Recognizes a phosphor-protein of 45kDa, identified as MyoD1. The epitope of this MAb maps between amino acid 180-189 in the C-terminal of mouse MyoD1 protein. It does not cross react with Myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occasionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilm’s tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and in rhabdomyosarcomas, neuroblastomas, Ewing’s sarcomas and alveolar soft part sarcomas.

Immunogen:

Recombinant mouse MyoD1 protein

Presentation:

Bioreactor Concentrate with 0.05% Azide

Specification:

Reacts with a 70kDa membrane-bound isozyme of Placental Alkaline Phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is highly specific for PLAP and shows no cross reaction with other isozymes of alkaline phosphatase. Anti-PLAP reacts with germ cell tumors and can discriminate between these and other neoplasms. Somatic neoplasms e.g. breast, gastrointestinal, prostatic and urinary cancers may also immunoreact with antibodies to PLAP. Anti PLAP positive in conjunction with anti-keratin positive, but they regularly fail to stain with anti-EMA, whereas most carcinomas stain with anti-EMA. Anti-PLAP as been useful in the diagnosis of gestational trophoblastic disease.

Presentation:

Bioreactor Concentrate with 0.05% Azide

Specification:

Keratin 20 / cytokeratin20 (CK20) is a type-I keratin which is primarily expressed in gastric and intestinal epithelium, urothelium, and Merkel-cells. CK20 is expressed in adenocarcinomas of the colon, stomach, pancreas and the bile system. CK20 is also present in mucinous ovarian tumours, transitional-cell and Merkel-cell carcinomas.

Immunogen:

Peptide derived from C-terminal region of human cytokeratin 20

Presentation:

monospecific rabbit clonal antibody in 20 mM Tris-HCl, pH 8.0 with 20 mg/ml BSA and 0.05% NaN3

Specification:

Anti-EMA antibody is a useful marker for staining many carcinomas. It stains normal and neoplastic cells from various tissues, including mammary epithelium, sweat glands and squamous epithelium. Hepatocellular carcinoma, adrenal carcinoma and embryonal carcinomas are consistently EMA negative, so keratin positivity with negative EMA favors one of these tumors. EMA is frequently positive in meningioma, which can be useful when distinguishing it from other intracranial neoplasms, e.g. Schwannomas. The absence of EMA can also be of value since negative EMA staining is characteristic of some tumors including adrenal carcinoma, seminomas, paraganglioma and hepatoma.

Immunogen:

Delipidated extract of human milk fat globule membranes

Presentation:

Bioreactor Concentrate with 0.05% Azide

Specification:

In Western blotting, anti-EMA (MUC-1) recognizes proteins in MW range of 265-400kDa, identified as different glycoforms of EMA. This MAb reacts with the DTRP epitope in the tandem repeats. The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. EMA may provide a protective layer on epithelial cells against bacterial and enzyme attack. The beta subunit contains a C-terminal domain, which is involved in cell signaling, through phosphorylation’s and protein-protein interactions. In immunohistochemical assays, it superbly stains routine formalin/paraffin carcinoma tissues. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.

Immunogen:

Human milk fat globule membranes

Presentation:

Bioreactor Concentrate with 0.05% Azide

Specification:

Recognizes a protein of 150kDa, which is identified as the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120–150kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70–80kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This MAb recognizes only the 150kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.

Immunogen:

Crude human uterus extract

Presentation:

Bioreactor Concentrate with 0.05% Azide

Specification:

A disulphide-linked heterodimer, consisting of mb-1 (or CD79a) and B29 (or CD79b) polypeptides, is non-covalently associated with membrane-bound immunoglobulins on B cells. This complex of mb-1 and B29 polypeptides and immunoglobulin constitute the B cell Ag receptor. CD79a first appears at pre B cell stage, early in maturation, and persists until the plasma cell stage where it is found as an intracellular component. CD79a is found in the majority of acute leukemias of precursor B cell type, in B cell lines, B cell lymphomas, and in some myelomas. It is not present in myeloid or T cell lines. It is superb for staining formalin-fixed, paraffin-embedded B-cell lymphomas.

Immunogen:

A synthetic peptide corresponding to aa 202-216 (GTYQDVGSLNIADVQ) of human CD79a protein

Presentation:

Bioreactor Concentrate with 0.05% Azide.