دسته: آنتی بادیهای ایمونوهیستوشیمی

Specification:

Recognizes a single chain glycoprotein of 105/120kDa, identified as CD30/Ki-1. CD30 is synthesized as a 90kDa precursor, which is processed in the Golgi complex into a membrane-bound phosphorylated mature 105/120kDa glycoprotein. In Hodgkin’s disease, CD30/Ki-1 antigen is expressed by mononuclear-Hodgkin and multinucleated Reed-Sternberg cells. It is also expressed by the tumor cells of a majority of anaplastic large cell lymphomas as well as by a varying proportion of activated T and B cells. This MAb distinguishes large cell lymphomas derived from activated lymphoid cells from histiocytic malignancies and lymphomas derived from resting and precursor lymphoid cells or from anaplastic carcinomas. About one third of the Ki-1 positive lymphomas lack the leukocyte common antigen (CD45).

Immunogen:

L428 cells

Presentation:

Bioreactor Concentrate with 0.05% Azide

Specification:

This antibody has been used for evaluating the primary site of a metastatic carcinoma of unknown origin and distinguishing between benign and malignant lesions. It is believe that CA15-3 reacts primarily with the DF3-antigen, a 300 kDa mucinlike glycoprotein present on the apical border of secretory mammary epithelial cells. CA15-3 has been detected with immunohistochemistry in a wide spectrum of carcinomas, including Breast Carcinomas (ductal and lobular), Sarcomas (Synovial Sarcoma and Malignant Fibrous Histiocytomas), and Lung Carcinomas. CA15-3 can be used as a supplementary marker for epithelial differentiation. CA15-3 does not stain Melanomas or Ewing’s Sarcomas. Approximately 30% of Hepatocellular Carcinomas are positive for CA15-3.

Presentation:

CA15-3 is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.

Specification:

PDCD-1 (Programmed cell death-1 protein), also designated CD279m is a type I transmembrane receptor and member of the immunoglobulin gene superfamily. It is expressed on activated T-cells, B-cells and myeloid cells. Anti-PDCD-1 is a marker of angioimmunoblastic lymphoma and suggest a unique cell of origin for this neoplasm. Unlike CD10 and BCL6, PDCD-1 is expressed by few B-cells, so anti-PDCD-1 expression provides evidence that angioimmunoblastic lymphoma is a neoplasm derived from germinal center-associated T-cells.

Presentation:

Bioreactor Concentrate with 0.05% BSA % 0.05% Azide.

Specification:

Anti CA-125 antibody reacts with epitheloid malignancies of the ovary, papillary serous carcinoma of the cervix, adenocarcinoma of the endometrium, clear cell adenocarcinoma of the bladder, and epitheloid mesothelioma. The antigen is formalin resistant, permitting the detection of ovarian cancer by immunohistochemistry, although serum assays for this protein are widely used to monitor ovarian cancer. Anti CA-125 also reacts with antigens in seminal vesicle carcinoma.

Presentation:

Anti-CA-125 is a mouse monoclonal antibody from ascites diluted in Tris- buffered saline, pH 7.3 – 7.7, with protein base, and preserved with sodium azide.

Specification:

This antibody recognizes a cluster of proteins between 70-80kDa, identified as tyrosinase. Occasionally a minor band at 55kDa is also detected. This MAb shows no cross-reaction with MAGE-1 and tyrosinase-related protein 1, TRP-1/gp75. Tyrosinase is a copper-containing metalloglycoprotein that catalyzes several steps in the melanin pigment biosynthetic pathway: the hydroxylation of tyrosine to L-3,4-dihydroxy-phenylalanine (dopa), and the subsequent oxidation of dopa to dopaquinone. Mutations of the tyrosinase gene occur in various forms of albinism. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Staining of melanomas with this MAb shows tyrosinase in melanotic as well as amelanotic variants. This MAb is a useful marker for melanocytes and melanomas.

Immunogen:

Recombinant tyrosinase protein

Presentation:

Bioreactor Concentrate with 0.05% Azide

Specification:

This antibody recognizes cytokeratin polypeptides ranging from 40 through 58kD, corresponding to cytokeratin’s 5, 6, 8,17, and 19. It shows a broad pattern of reactivity with human epithelial tissues from simple glandular epithelia to stratified squamous epithelia, like epidermis, mammary gland ducts, and tracheal epithelium.

Immunogen:

Crude extract of splenic cells from a nude mouse engrafted with MCF-7 cells

Presentation:

This antibody is supplied as tissue culture supernatant containing sodium azide as a preservative

Specification:

SCLC-CD2; epithelial antigen, EGP-2. MOC-31 reacts with an epithelial antigen of 40kD present on most normal and malignant epithelia.MOC-31 has been assigned to a group of antibodies known as SCLC-Cluster 2 which react with an epithelial antigen determined at the Second International Workshop on Small Cell Lung Cancer (SCLC)Antigens. In normal tissues, clone MOC-31 reacts with glands and hair shafts in skin, pancreatic exocrine and endocrine glands, epithelia of the digestive and respiratory tract, including sero-mucous glands. MOC-31 stains epithelia of kidney, endometrium, breast, liver, prostate, pancreatic acini, slets and ducts. No reactivity is observed with adrenal, ovary, brain, peripheral nerve, ganglion cells, peripheral blood and bone marrow. Lung-derived tumours such as squamous cell carcinomas, adenomas, small cell lung carcinomas, carcinoids, adenocystic carcinomas, carcinosarcoma, mucoepidermal carcinoma and pleomorphic adenoma stain positively with MOC-31.Also,colon adenocarcinoma, gastric adenocarcinoma, esophagealgastric adenocarcinoma, some bladder transitional cell carcinomas, prostate adenocarcinoma, testicular yolk tumours, uterine adenomatoid tumours, ovary mucinous/endometrial cancers, serous carcinoma of the ovary, some breast carcinomas, thyroid papillary carcinoma and thyroid medullary carcinomas may be positive.

Immunogen:

Neuraminidase treated cells from a variant small cell lung carcinoma cell line (GLS-1)

Presentation:

Bioreactor Concentrate with 0.05% Azide

Specification:

There are at least nine members of the paired box (PAX) gene family whose protein products are transcription factors involved in development. The conserved paired box DNA-binding domain is found in the N-terminal region of Pax proteins. An octomer and homeodomain sequence are conserved in the centre of the proteins. The Ser/Thr/ Pro-rich region in the Cterminal portion contains a conserved 100 amino acid transactivating domain. One of the best studied Pax family members, Pax-5 influences the expression of several B-cell-specific genes, such as CD 19 and CD 20. Pax-5 is expressed primarily in pro-, pre-, and mature B cells, but not in plasma cells. Interestingly, Pax-5 mRNA is transiently detected in the mesencephalon and spinal cord during embryogenesis. Expression then shifts to the fetal liver and correlates with the onset of B lymphopoiesis. PAX-5 has been found to be important in both B cell and nervous system development.

Immunogen:

Human PAX-5 aa. 151-306

Presentation:

0.25 mg/ml in aqueous buffered solution containing BSA, glycerol, and ≤ 0.09% sodium azide