دسته: مونوکلونال

Specification:

Recognizes a protein of 150kDa, which is identified as the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120–150kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70–80kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This MAb recognizes only the 150kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.

Immunogen:

Crude human uterus extract

Presentation:

Bioreactor Concentrate with 0.05% Azide

Specification:

A disulphide-linked heterodimer, consisting of mb-1 (or CD79a) and B29 (or CD79b) polypeptides, is non-covalently associated with membrane-bound immunoglobulins on B cells. This complex of mb-1 and B29 polypeptides and immunoglobulin constitute the B cell Ag receptor. CD79a first appears at pre B cell stage, early in maturation, and persists until the plasma cell stage where it is found as an intracellular component. CD79a is found in the majority of acute leukemias of precursor B cell type, in B cell lines, B cell lymphomas, and in some myelomas. It is not present in myeloid or T cell lines. It is superb for staining formalin-fixed, paraffin-embedded B-cell lymphomas.

Immunogen:

A synthetic peptide corresponding to aa 202-216 (GTYQDVGSLNIADVQ) of human CD79a protein

Presentation:

Bioreactor Concentrate with 0.05% Azide.

Specification:

Napsin A is an aspartic protease with a molecular weight of approximately 38 kDa, expressed in type-II pneumocytes and is involved in the N- and C-terminal processing of proSP-B in type-II pneumocytes. TAO2 has been shown to be identical with Napsin A. There is also a Napsin B gene which is transcribed exclusively in cells related to the immune system but lacks a stop codon and may represent a transcribed pseudogene. Napsin A is predominantly expressed in the lung and kidney. In the lung, Napsin A is expressed in alveolar type II pneumocytes, regulated by TTF-1, and is involved in the generation of the surfactant protein B. Intra-alveolar macrophages contain Napsin A as a result of phagocytosis. In the kidney, Napsin A is expressed in the proximal tubules, where it is involved in lysosomal protein catabolism.

Presentation:

Liquid tissue culture supernatant containing 15mM sodium azide

Specification:

ECAD (syn, CD325), 120 kDA, chromosome 16q22.1 (CDH1 gene), is a critical regulator of epithelial junction formation. It interacts with the cytoskeleton through several associated proteins. The ECAD internal domain binds with alpha, beta, gamma and p120 catenin’s to anchor the ECAD complex to the actin cytoskeleton of the cell. ECAD is expressed in virtually all epithelial cells except for adrenocortical cells. The expression in liver cells is weaker than in most other epithelia. ECAD is also expressed in melanocytes (adhering to squamous epithelial cells).

Presentation:

Tissue culture supernatant containing 15mM sodium azide

Specification:

Cytokeratin’s (epithelial keratins) are an important component of the intermediate filament system. They are mainly insoluble molecules playing an important role in cellular mechanics. There are two types of cytokeratin’s: a) type I: (9-20) keratins that are relatively acidic and bearing a small molecular weight (40-56.5 kDa) and b) type II: (1-8) that are relatively basic-neutral of larger molecular weight (53-67 kDa). Proliferating cells have a substantial pool of two soluble cytokeratin’s, namely CK8 and CK18 and their concentration is high during the G2-M phase of the cell cycle. During apoptosis, CK18 are cleaved by caspases at position Asp396 producing relatively stable fragments.

Presentation:

Tissue culture supernatant containing 15mM sodium azide

Specification:

NK-1 antibody marks a subset of lymphocytes known as natural killer (NK) cells. Follicular center cell lymphomas often contain many NK cells within the neoplastic follicles. NK-1 also stains neuroendocrine cells and their derived tumors, including carcinoid tumor, and medulloblastoma. NK-1 reportedly also reacts with a variety of cell types in non-lymphoid tissues, including neurofibroma, ganglioneuroma, and prostate carcinoma.

Immunogen:

Human peripheral blood mononuclear cells

Presentation:

Bioreactor Concentrate with 0.05% Azide

Specification:

Tenascin C is a multifunctional, disulfide-linkedhexameric extracellular matrix glycoprotein expressed in association with mesenchymal epithelial interactions during development and in the neo-vasculature and stroma of undifferentiated tumors. In adults, it is restricted to certain epithelial-stromal interfaces and increases markedly in hyper-proliferative diseases and in stroma of many neoplasms, including gliomas, breast, squamous and lung carcinomas.

Immunogen:

Human breast carcinoma

Presentation:

Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide

Specification:

Anti-CD45RO (T-Cell, Pan) antibody reacts with thymocytes and activated T-cells, but only on a subpopulation of resting Tcells. This antibody shows no reactivity with B-cells making it a good marker for T-cell tumours. In addition, granulocytes and monocytes are  also labelled with this antibody. T-Cell, Pan has been designated as CD45RO at The International Leukocyte Typing Workshop.

Presentation:

Mouse monoclonal antibody from supernatant diluted in tris buffered saline, pH 7.3-7.7, with protein base, and preserved with sodium azide

Specification:

ZAP-70 is a 70kDa protein tyrosine kinase found in T-cells and natural killer cells. Control of this protein translation is via the IgVH gene. In Western blotting of whole cell lysates of normal peripheral blood mononuclear cells, the antibody labels a band corresponding to ZAP-70. In Western blotting of whole cell lysates of CD19-positive purified leukemia cells from patients with lg-unmutated and lg-mutated CLL, the antibody labels a band corresponding to ZAP-70 in the Ig-unmutated CLL samples, whereas no band is observed in the Ig-mutated CLL samples. In Western blotting of cell lysates of Jurkat cells (T-lymphoblastic cell line), the antibody labels a band of 70kDa protein. In Western blotting of cell lysates of A431 cells (carcinoma cell line), no band is observed. ZAP-70 protein is expressed in leukemic cells of approximately 25% of chronic lymphocytic leukemia (CLL) cases as well. Anti-ZAP-70 expression is an excellent surrogate marker for the distinction between the g-mutated (antiZAP-70 negative) and Ig-unmutated (anti-ZAP-70 positive) CLL subtypes and can identify patient groups with divergent clinical courses. The anti-ZAP-70 positive Ig-unmutated CLL cases have been shown to have a poorer prognosis.

Immunogen:

Recombinant ZAP-70 protein including residues 1-254 and encompassing SH2 domains of human ZAP-70

Presentation:

Bioreactor Concentrate with 0.05% Azide