آنتی بادی مونوکلونال فلوسایتومتری CD16-FITC ، کلون 3G8

Name: Flow Cytometry Antibody CD16-FITC, Clone 3G8

• Antibody CD16-FITC is a monoclonal antibody (mAb) labelled with fluorescein isothiocyanate (FITC) designed for use as a direct immunofluorescence reagent in the identification and enumeration of cells which express the CD16 antigen by flow cytometry (FC).This reagent must be used by flow cytometry qualified personal.

SUMMARY AND EXPLANATION

Human lymphocytes may be classified in three main populations according to their biological function and their cell surface antigen expression: T lymphocytes, B lymphocytes and natural killer cells (NK). NK cells (CD3-CD16+) mediate cytotoxicity against certain tumors and virus-infected cells. NK-mediated cytotoxicity does not require class I or class II major histocompatibility complex (MHC) molecules to be present on the target cells. The CYT-16F6 mAb recognizes the CD16 antigen present on NK cells, macrophages and neutrophils, and can therefore be used in the characterization studies for immunophenotyping of leucocytes. These studies are widely applied for monitoring of the immunologic status of post-transplant patients and in the characterization and follow-up of immunodeficiencies, autoimmune diseases, leukemia etc.

REAGENT COMPOSITION

Purified monoclonal CD16 Antibody conjugated with fluorescein isothiocyanate (FITC), is supplied in phosphate-buffered saline (PBS) containing 1% (m/v) BSA and 0.09% (m/v) sodium azide.

• Clone: 3G8
• Isotype: IgG1
• Purification: Affinity chromatography
• Amount per 1 ml vial: 200 tests (5 ml mAb to 106 cells)
• Reagents are not considered sterile.