آنتی بادی مونوکلونال فلوسایتومتری CD19-FITC ، کلون HIB19

Name: Flow Cytometry Antibody CD19-FITC (HIB19), Clone HIB19

• Antibody CD19 reagent is a monoclonal antibody (mAb) conjugated with different fluorochromes (see table) and designed for use as a direct immunofluorescence reagent in the identification and enumeration of cells which express the CD19 antigen by Flow Cytometry (FC). This reagent must be used by FC qualified personal.

SUMMARY AND EXPLANATION

The anti-CD19 mAb recognizes the CD19 antigen present in B cells and can therefore be used in the characterization studies for immunophenotyping of lymphocytes. The CD19 antigen appears early during B cell maturation, it is expressed continuously throughout the remainder of B lymphocyte differentiation including the resting and the activated mature peripheral blood or lymphoid tissue B lymphocyte, and it is lost at the last stage of B lymphocyte differentiation, the plasma cell. The CD19 expression is maintained in B-lineage cells that have undergone neoplastic transformation. Antibodies to CD19 are widely applied in the characterization and follow-up of acute and chronic lymphoproliferative disorders. The B lymphocyte (CD19+) count is generally expressed as a percentage of the total amount of lymphocytes or leucocytes present in the sample which can itself be determined by FC based on its typical pattern of FSC/SSC (size/granularity or complexity). Because each flow cytometer has different operating characteristics each laboratory must determine its optimal operating procedure.

REAGENT COMPOSITION

Purified monoclonal CD19 antibody conjugated with different fluorochromes (see table above) is supplied in phosphatebuffered saline (PBS) containing 0,09% (m/v) sodium azide.

• Purification: Affinity chromatography
• Size: 200 or 50 tests per vial (see table above)
• Usage: 5 or 3 µl mAb per determination (see table above)
• Reagents are not considered sterile.