آنتی بادی مونوکلونال فلوسایتومتری CD7-FITC ، کلون B-B7

Name: Flow Cytometry Antibody CD7-FITC, Clone B-B7

• Antibody CD7-FITC is a monoclonal antibody (mAb) labelled with fluorescein isothiocyanate (FITC) designed for flow cytometry (FC) use as a direct immunofluorescence reagent in the identification and enumeration of CD7 antigen-expressing cells. The CD7 mAb is considered essential for the initial evaluation of T-cell acute lymphoblastic leukaemias (T-ALL) and T-cell chronic leukaemias together with a panel of other antibodies. This reagent must be used by flow cytometry qualified personal.

SUMMARY AND EXPLANATION

Human lymphocytes may be classified in three main populations according to their biological function and their cell surface antigen expression (T lymphocytes, B lymphocytes and natural killer (NK) cells. CYT-7F5 mAb recognizes CD7 antigen, a 40KDa glycoprotein expressed on thymocytes, mature T cells, natural killer cells, pluripotent hematopoietic stem cells, and progenitors of lymphoid and myeloid cells. CD7 is one of the earliest Tcell-specific antigens to be expressed in lymphocytes, and the only early marker to persist throughout differentiation. Analysis of the expression of the CD7 antigen as a T cell line marker, in combination with those CD3 and CD5 antigens, is useful in the diagnosis of T cell proliferative syndromes such as T-ALL and T prolymphocyte T cell leukaemias. The absence of expression of CD7, or its abnormal expression, can provide useful information in the case of Sezary’s syndrome or chronic lymphoid leukaemia. The CD7 antigen can be expressed by certain acute immature myeloid leukaemias.

REAGENT COMPOSITION

The purified monoclonal CD7 antibody conjugated with fluorescein isothiocyanate (FITC) is supplied in phosphate-buffered saline (PBS) containing 0,09% sodium azide.

• Clone: B-B7.
• Isotype: IgG1.
• Amount per 1 ml vial: 200 tests (5 µL mAb per determination).
• Reagent is considered non-sterile.