آنتی بادی مونوکلونال فلوسایتومتری CD8-PE ، کلون UCHT-4

Name: Flow Cytometry Antibody CD8-PE, Clone UCHT-4

• Antibody CD8 reagent is a monoclonal antibody (mAb) labelled with different fluorochromes (see table above) and designed for use as a direct immunofluorescence reagent in the identification and enumeration of cells which express the CD8 antigen by Flow Cytometry (FC). This reagent must be used by flow cytometry qualified personal.

SUMMARY AND EXPLANATION

Human lymphocytes may be classified in three main populations according to their biological function and their cell surface antigen expression: T lymphocytes, B lymphocytes and natural killer cells (NK). T lymphocytes (CD3+), the precursors of which originate in the bone marrow and then migrate and mature in the thymus, can be subdivided as well in functionally different populations. The most clearly defined of these are helper/inducer T cells (CD3+CD4+) and suppressor/cytotoxic T cells (CD3+CD8+). T cells produce no antibodies and are the mediators of cell immunity. The anti-CD8 mAb recognizes the CD8 antigen present in suppressor/cytotoxic T cells (CD3+CD8+) and can therefore be used in the characterization studies for immunophenotyping of lymphocytes. These studies are widely applied for monitoring of the immunologic status of post-transplant patients and in the characterization and follow-up of immunodeficiencies, autoimmune diseases, leukemia etc (3,4).

REAGENT COMPOSITION

Purified monoclonal CD8 Antibody labelled with different fluorochromes (see table above) and supplied in phosphate buffered saline with 0,09% (m/v) sodium azide.

• Clone: UCHT-4
• Isotype: Mouse / IgG2a
• Purification: Affinity chromatography
• Amount per vial: 200 tests (5 ml mAb to 106 cells)
• Reagents are not considered sterile.