Name: Flow Cytometry Antibody CD180-APC-C750, Clone G28-8
- Antibody CYT-180AC750 is a monoclonal antibody (mAb) labelled with tandem allophycocyanin-C750 (APC-C750TM) designed for use as a direct immunofluorescence reagent in the identification and enumeration of cells which express the CD180 antigen by flow cytometry. This reagent must be used by flow cytometry qualified personnel.
SUMMARY AND EXPLANATION
CD180 (RP105), belongs to the toll-like receptor (TLR) family and is expressed on most B cells, monocytes/macrophages, and dendritic cells. It is a 105 kDa protein consisting of extracellular leucine-rich repeats and a short cytoplasmic tail. MD-1, a secretory protein, is associated with CD180 at the cell surface and, in conjunction with TLR4, recognizes bacterial lipopolysaccharide. Ligation of CD180 by monoclonal antibodies leads to B cell activation.
REAGENT COMPOSITION
Purified monoclonal CD180 antibody conjugated with tandem allophycocyanin-C750 (APC-C750TM), supplied in phosphate buffered saline with 0,09% sodium azide.
- Clone: G28-8.
- Isotype: Mouse / IgG1.
- Amount per vial: 50 tests. 3 µl mAb per determination.
- Reagents are not considered sterile.
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