Name: Flow Cytometry Antibody IgM-PerCP-Cyanine5.5, Clone MHM-88.
Antibody IgM-PerCP-Cyanine5.5 is designed for flow cytometry use as a direct immunofluorescence reagent in the identification and enumeration of IgM expressing cells.
Monomeric IgM is always the first form and isotype of Ig generated by naive B cells. Following its initial activation by antigen, the naive B cell proliferates and differentiates, and its progeny produce the pentameric secreted form of IgM. Thus, it is IgM antibodies that are expressed first in any primary immune response. IgM antibodies comprise only about 5–10% of normal serum Ig.
Although the bulk of IgM is found in the blood, if vascular permeability has been increased in a local area by the release of vasoactive compounds during an inflammatory response, IgM antibodies can exit the blood and enter the tissues to reach sites of infection. Although the concentration of IgM antibodies in the external secretions is very low compared to that of IgA, secretory IgM antibodies do play an important role in mucosal humoral immunity.
CYT-IGMC2 should be used with BulkLysisTM (CYT-BL) buffer that allows you to stain a great number of cells and analyze low frequency subsets, as IgM+ memory B cells and plasma cells. If another lysing protocol is used, two pre-staining washing steps using PBS + 1% (m/v) BSA + 0,09% (m/v) sodium azide must be performed.
This reagent must be used by flow cytometry qualified personnel.
Purified monoclonal anti-IgM antibody (mAb) conjugated with Peridin chlorophyll protein-Cyanine 5.5 (PerCP-Cyanine5.5), supplied in phosphate buffered saline with 0,09% (m/v) sodium azide and BSA 1% (m/v).
- Clone: MHM-88.
- Isotype: Mouse IgG1.
- Amount per vial: 25 tests using 3µL per 106 cells