| REF | BU-003 |
|---|
Phosphate buffered saline (PBS) is a commonly used buffer for biological and IHC applications. PBS is used for all processes that require a salt concentration close to the physiological state, adapted to the pH value required in histology or in cell culture techniques. PBS is mainly used as a wash buffer in histology, serology, cell culture, biochemical assays (ELISA, RIA) and protein purification. Phosphate buffers should not be used in enzyme assays where competition for phosphate groups or complexation with a metal ion is essential for enzyme activation.
