آنتی بادی مونوکلونال فلوسایتومتری CD23-FITC ، کلون EBVCS-5

Name: Flow Cytometry Antibody CD23-FITC, Clone EBVCS-5

• Antibody CD23-FITC is a monoclonal antibody (mAb) labelled with Fluorescein isothiocyanate (FITC) designed for flow cytometry (FC) use as a direct immunofluorescence reagent in the identification and enumeration of CD23 antigen-expressing cells.

SUMMARY AND EXPLANATION

FC is a powerful tool in analytical and quantitative characterization of cells which provides rapid and multiparametric analysis of heterogeneous cell populations on a cell-by-cell basis. Flow cytometry is performed on cell suspension after incubating it with fluorescent-labelled antibodies directed against specific cellular proteins. Positive cells relative fluorescence intensity indicates the amount of antibody bonded to specific cell sites providing information about antigen expression.

Human lymphocytes may be classified in three main populations according to their biological function and their cell surface antigen expression (T lymphocytes, B lymphocytes and natural killer (NK) cells). CD23 antigen is a type II integral membrane glycoprotein. It is a member of the Ig family, expressed on most mature B cells, mantle zone B cells (but not in proliferating germinal center cells), follicular dendritic cells, monocytes, eosinophils, Langerhans cells, and a subset of T cells (10-15% of tonsillar T cells). CD23 is the low affinity receptor for IgE and plays important roles in the regulation of IgE responses; it is responsible for interaction with many of its known ligands, including IgE, CD21, major histocompatibility complex (MHC) class II and integrins. Anti CD23 antibody is used in flow cytometry for the immunophenotyping of B cell lineage leukemias and lymphomas. The diagnostic and prognostic utility of CD23 expression has been widely studied in B cell chronic lymphocytic leukemia. CD23 is overexpressed in some B-chronic lymphoproliferative diseases, mantle cell lymphoma, small cell lymphocytic lymphoma, follicular dendritic cell sarcoma and plasmacytomas with abnormalities on chromosome 11, whereas is generally absent from follicular lymphoma cells and acute lymphoblastic leukaemia cells.

REAGENT COMPOSITION

The purified monoclonal CD23 Antibody conjugated with Fluorescein Isothiocyanate (FITC) is supplied in phosphate-buffered saline (PBS) containing 0.1% sodium azide.

• Clone: EBVCS-5
• Isotype: IgG1.
• Amount per vial: 100 tests (5 µl mAb per determination).
• Reagent is considered non-sterile.