آنتی بادیهای ایمونوهیستوشیمی

Name: Mouse anti-human Smooth Muscle Actin (SMA) Monoclonal Antibody (Clone 1A4) 

Description and aplications: Actin is a major component of the cytoskeleton and is present in every cell type. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-Smooth Muscle Actin antibody does not stain cardiac or skeletal muscle;
however, it will stain myofibroblasts and myoepithelial cells. This antibody could be used together with Muscle Specific Actin to distinguish leiomyosarcoma from rhabdomyosarcoma. In most cases of rhabdomyosarcoma, this antibody gives negative results whereas Muscle Specific Actin is positive in the rhabdomyoblasts. Leiomyosarcomas are positive with both Muscle Specific Actin and Smooth Muscle Actin antibodies.

Composition: Anti-human SMA mouse monoclonal antibody purified from serum and prepared in 10mM PBS, pH
7.4, with 0.2% BSA and 0.09% sodium azide

Immunogen: N-Terminal decapeptide of alpha smooth muscle isoform of actin; acetylated at the Nterminus.

Name: Rabitt anti-human SOX9 Antibody (Clon EP317) 

Description and aplications: SOX9 is a nuclear transcription factor involved in the differentiation of the chondrocytes and the skeleton as well as of sexual development. The factor is encoded by the gene with the same name located in the chromosome region 17q24.3. This gene encodes a peptide consisting of 509 amino acids containing a
domain homologous to SRY. The co-expression of the genes SOX9 and COL2A1 as well as the overexpression of the first, which is induced by the fibroblast growth factor of primordial chondrocytes, intervenes in the differentiation into mature chondrocytes. About the latter ones, SOX9 interacts additionally with the parathyroid hormone related protein (PTHRP) and helps in keeping its phenotype, inhibiting maturation of hypertrophic chondrocytes. In the determination of the sex of the product of fertilization, SOX9 intervenes together with the steroidogenic factor 1 (SF-1), most probably through SRY gene, functioning as an essential factor in the differentiation of Sertoli cells. The transient activation of the SRY gene of the Y chromosome initializes a set of interactions leading to the formation of the testicle from the undifferentiated gonad; so SOX9 plays a crucial role in this pathway. In fact, the SOX9 gene is overexpressed during the activation of the SRY gene with transfer of its protein from a cytoplasmic to a nuclear location. In contrast, in the female gonad, the SOX9 gene is regulated downwards.

Composition: anti-human SOX9 rabbit monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide

Name: Rabbit anti-human SOX-10 Monoclonal Antibody (Clone EP268) 

Description and aplications: SOX10 is a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. During development, SOX10 first appears in the forming neural crest and continues to be expressed in Schwann cells. It is important for differentiation, maturation and maintenance of Schwann cells and melanocytes. In normal tissues, SOX10 is expressed in Schwann cells and glial cells in the nervous system. It is also detected in melanocytes and epithelial cells of salivary gland and mammary gland. In tumor tissues, SOX10 labels melanoma and the tumor of neural crest origin. A recent study reported the expression of SOX10 in basal-like, unclassified triple-negative breast carcinoma. Thus, breast carcinoma must be considered in the differential diagnosis of melanoma for a SOX10-positive metastatic malignant neoplasm.

Composition: Anti-human SOX-10 rabbit monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide

Immunogen: A recombinant fragment corresponding to residues in human SOX10 protein.

Name: Mouse anti-Steroidogenic Factor 1 Monoclonal Antibody (clone N1665) 

Description and aplications: Steroidogenic factor 1 (SF-1), also known as Ad4BT (adrenal 4 binding protein) or NR5A1 (nuclear receptor subfamily, group 5, member 1) is a transcription factor that belongs to the nuclear receptor superfamily and functions as a key regulator in the development and function of the adrenal gland and the male reproductive system after the enzymatic control of the biosynthesis of corticosteroids and aldosterone. It also intervenes at pituitary level for the development of the gonads. SF-1 is encoded by the NR5A1 gene, located in the region q33 of chromosome 9. It is a gene of 22kb containing 7 exons. Mutations of the gene are responsible for the development of dysgenetic gonads and ovarian insufficiency in women or female gonads or ambiguous gonads in men. It is also responsible for the absence of puberty and infertility, especially in men.

Composition: Mouse anti-Steroidogenic Factor 1 (SF1) monoclonal antibody obtained from purified ascitic fluid and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Name: Mouse anti-human Tyrosinase Monoclonal Antibody (Clone T311) 

Description and aplications: Melanin synthesis by melanocytes comprises a cascade of reactions involving a family of enzymes. One of the key enzymes is tyrosinase. L-tyrosine is the initial substrate for melanin synthesis and its conversion to dopaquinone is catalysed by tyrosinase, the expression of which is therefore a marker of melanocytes and melanoma.
Tyrosinase deficiency is associated with various forms of albinism, specially oculocutaneous albinism. This antibody is useful for the identification of melanocytic tumours and it may be used in combination with other markers such as Melan A. Both markers are expressed in 80-100% of melanoma cases.

Composition: Anti-human Tyrosinase mouse monoclonal antibody purified from serum and prepared in 10mM PBS, pH
7.4, with 0.2% BSA and 0.09% sodium azide

Immunogen: Recombinant protein corresponding to the tyrosinase molecule.

Name: Mouse Anti-Human Uroplakin II Monoclonal Antibody (Clone BC21) 

Description and aplications: Uroplakin II (UP2) is a membrane-specialized protein of 15kDa that, along with the Uroplakins 1A, 1B and 3, compose the asymmetric membrane unit located in the apical cell plates of the human urothelium. UP2 is synthesized by urothelial cells in the final stage of differentiation. The synthesis of the protein is coded by the UPK2 gene located in the chromosome region 11q23.3. In normal tissues, the antibody specifically recognizes the UP2 in the surface layers of the normal urothelium, being negative in the rest of the human epithelia.

Composition: anti-Uroplakin 3 mouse monoclonal antibody obtained from supernatant culture and prediluted in a tris buffered solution pH 7.4 containing 0.375mM sodium azide solution as bacteriostatic and bactericidal.

Immunogen: Residues corresponding to amino acids 3650 of the human Uroplakin II

Name: Rabbit Anti-Human PRAME 1 Monoclonal Antibody (Clone EPR20330)

Description and aplications: The PReferentially expressed Antigen in MElanoma (PRAME), or LB33-E antigen, is part of the family of melanoma nuclear proteins recognized by cytolytic T lymphocytes and is encoded by a gene of the same
name (PRAME) located on chromosomal region 22q11.22. The PRAME protein has been characterized as a dominant inhibitor of the retinoic acid receptor, thus participating in the blockade of cell proliferation, differentiation or apoptosis induced by retinoic acid through the RARA, RARB and RARG receptors. Thus, overexpression of PRAME in tumor cells confers a survival advantage over normal cells. In melanomas, PRAME inhibition restores retinoic acid signaling an reinstates the sensitivity of tumor cells to the antiproliferative effects of this molecule.

Composition: Anti-human PRAME 1 rabbit monoclonal antibody obtained from ascitic and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Immunogen: N/A

Name: Mouse anti- Human Renal cell carcinoma marker (PN-15) Monoclonal Antibody

Description and aplications:This antibody detects the membrane glycoprotein gp200. In a normal kidney, gp200 is located along the brush border of the contoured and straight segments of the proximal tubule, as well as focally along the luminal surface of Bowman’s capsule, contiguous with the exit of the proximal tubule. Gp200 is also located on the luminal surface of the mammary ducts and lobes and the tubular epithelium of the epididymis, in the cytoplasm of parathyroid and parenchymal cells, and focally within the colloid of thyroid follicles.

Composition: Anti-human renal cell carcinoma marker (PN-15) mouse monoclonal antibody purified and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Immunogen: Microsomal fraction of human renal cortical tissue homogenate.