دسته: مونوکلونال

Specification:

Podoplanin is a transmembrane mucoprotein (38 kDa) recognized by the D2-40 monoclonal antibody. Podoplanin is selectively expressed in lymphatic endothelium as well as lymphangiomas, Kaposi sarcomas and in a subset of angiosarcomas with probable lymphatic differentiation. Podoplanin has also been shown to be expressed in epithelioid mesotheliomas hemangioblastomas and seminomas.

Presentation:

Anti-Podoplanin is a mouse monoclonal antibody from supernatant diluted in tris buffered saline, pH 7.3-7.7, with protein base, and preserved with sodium azide.

Specification:

CD7 antigen is a cell surface glycoprotein of 40 kD expressed on the surface of immature and mature T-cells as well as natural Killer (NK) cells. It is a member of the immunoglobulin gene superfamily and is the first T-cell lineage associated antigen to appear in T-cell ontogeny, being expressed in T-cell precursors (preceding CD2 expression), and in myeloid precursors, in fetal liver and bone marrow, and persisting in circulating mature T-cells. While its precise function is not known, it is suggested that the molecule functions as an Fc receptor for IgM. CD7 is the most consistently expressed T-cell antigen in lymphoblastic lymphomas/ leukemias and is therefore anti-CD7 is a useful marker in the identification of such neoplastic proliferations. In mature post-thymic T-cell neoplasms, CD7 is the most common pan-T antigen to be aberrantly expressed, which is a useful pointer to a neoplastic T-cell process. CD7 has been shown to be immunoexpressed in 85% of mature peripheral T-cells, the majority of post-thymic T-cells, NK cells, T-cell lymphoblastic leukemia/lymphoma, acute myeloid leukemia, and chronic myelogenous leukemia, CD7 is conspicuously absent in adult T-cell leukemia/lymphoma and is not expressed in mycosis fungoides.

Immunogen:

A synthetic peptide corresponding to residues of human CD7 protein

Presentation:

Antibody in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide

Specification:

 p63 consists of two major isoforms -TAp63 and ΔNp63. These isoforms differ in the structure of the N-terminal domains. The TAp63 isoform (identified by anti-p63 antibody) contains a transactivation- competent ‘TA’domain with homology to p53, which regulates the expression of the growth -inhibitor y genes. In contrast, ΔNp63 isoform (identified by anti-p40 antibody) contains an alternative transcriptionally- inactive ‘ΔN’ domain, which antagonizes the activity of TAp63 and p53. The p40 (clone ZR8) recognizes exclusively ΔNp63 but not TAp63. p40 is a squamous cell carcinoma ‘specific’ antibody. It reacts with the vast majority of cases of squamous cell carcinomas of various origins, but not with adenocarcinomas. It is particularly useful in differentiating lung squamous cell carcinoma from lung adenocarcinoma. p40 antibody can also be used as an alternative basal cell/myoepithelial cell marker, which has similar sensitivity and specificity as that of p63 antibody. Therefore, p40 antibody may also be used as an alternative immunohistochemical marker for determining prostate adenocarcinoma vs. benign prostate glands and for determining breast intraductal carcinoma v s. invasive breast ductal carcinoma.

Immunogen:

Synthesized polypeptides from N-terminal domain of p63

Presentation:

Purified antibody from in 0.2% BSA and 15mM sodium azide.

 Specification:

CD33 (gp67, or siglec-3) is a 67 kDa glycosylated transmembrane protein that is a member of the sialic acid–binding immunoglobulin-like lectin (siglec) family. The genomic locus of this protein has been mapped to chromosome 19q13.1-3.5. In maturing granulocytic cells, there is progressive downregulation of CD33 from the blast stage to mature neutrophils. However, in monocytes and macrophages/histiocytes, strong expression of CD33 is maintained throughout maturation. Therefore, the positive control tissue should be bone marrow myeloid cells (especially myeloid precursors), liver Kupffer cells, lung alveolar macrophages, or placental syncytiotrophoblasts. Detection of CD33 using monoclonal antibodies has been a critical component in immunophenotyping acute leukemias, particularly acute myeloid leukemias. This anti-CD33 may be particularly advantageous for cases of acute myeloid leukemia, minimally differentiated (AML-M0) and acute monocytic leukemia (AML-M5), in which other paraffin section markers of myeloid differentiation (such as antimyeloperoxidase) may be negative. However, anti-CD33 staining cannot be used in isolation and must be correlated with other myeloid and lymphoid markers because cases of myeloid antigen–positive acute lymphoblastic leukemia may show bona fide CD33 expression.

Presentation:

Antibody diluted in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide

Specification:

Transcription factors containing the POU homeo domain have been shown to be important regulators of tissue-specific gene expression in lymphoid and pituitary differentiation and in early mammalian development. POU domain proteins contain a bipartite DNA-binding domain divided by a flexible linker that enables them to adopt various monomer configurations on DNA. The versatility of POU protein operation is additionally conferred at the dimerization level. Oct-3 (also known as Oct-4) is a mammalian POU transcription factor expressed by early embryo cells and germ cells. Oct-3/4 is essential for the identity of the pluripotential founder cell population in the mammalian embryo. A critical amount of Oct3/4 is required to sustain stem-cell self renewal, and up or down regulation induce divergent developmental programs. Two isoforms of Oct-3,termed Oct-3A and Oct-3B, are generated by alternative splicing. The gene which encodes Oct-3/4 maps to human chromosome 6p21.3. Oct-3/4 (C-10) is recommended for detection of Oct-3A (Oct-4) and Oct-3B of mouse, rat and human origin by Western Blotting, immunoprecipitation, immunofluorescence, and paraffin immunohistochemistry.

Immunogen:

Amino acids1-134 of Oct-3/4 of human origin

Presentation:

Purified antibody in 0.2 % BSA and 15mM sodium azide