مونوکلونال

Name: Prolactin Antibody clone EP193

Description and applications: Prolactin is a peptide hormone secreted by the anterior pituitary that is necessary for the proliferation and differentiation of the mammary glands. Prolactin also acts in a cytokine-like manner and as an important regulator of the immune system. Prolactin has important cell cycle related functions as a growth, differentiating and antiapoptotic factor. Prolactin is secreted by lactotrophs in the anterior pituitary. Prolactin producing cells make up approximately 20 percent of the pituitary. Elevated counts of these cells have been observed in pregnant women, newborns and in multiparous women. An antibody to prolactin is useful for the identification of pituitary tumors.

Composition: Anti-human Prolactin rabbit monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide

Immunogen: A synthetic peptide corresponding to residues of human Prolactin protein

Name: PSA antibody Clone ER-PR8

Description and applications: PSA is an antigen present in prostatic tissue and in the majority of prostatic carcinomas. This antibody recognizes primary and metastatic prostatic neoplasms and rarely in tumors of nonprostatic origin. These include breast and a minority of salivary gland tumors. The antigen is a 33-34 kD glycoprotein that is restricted to epithelial cells of the prostate. Because of the high specificity of PSA for prostate glandular epithelium, the antibody is useful to identify prostatic carcinomas of the prostate and their frequent infiltration of the surrounding organs (eg .: rectum and urinary bladder). Rectal adenocarcinomas, urothelial carcinoma or adenocarcinoma of the urinary bladder did not express PSA. The loss of immunoreactivity for PSA which can occur in poorly differentiated neoplasms cannot completely exclude the diagnosis of prostate cancer with a negative PSA. Another important use is for differential diagnosis and determine the origin of the primary tumor with metastases of unknown origin, especially lymph node and bone. PSA can be used together with the Prostein, which has at least the same sensitivity and is slightly more specific tha PSA.

Composition: anti-human PSA mouse monoclonal antibody purified from supernatant diluted in tris bffered saline, pH 7.3-7.7, with protein base, and preserved with sodium azide.

Name: Prostatic Acid Phosphatase Antibody Clone PASE/4LJ

Description and Applications: Anti-PSAP reacts with prostatic acid phosphatase in the glandular epithelium of normal and hyperplastic prostate, carcinoma of the prostate and metastatic cells of prostatic carcinoma. This marker may be helpful in pinpointing the site of origin in cases of metastatic carcinoma of the prostate, and is considered a more sensitive marker than PSA. However, it also offers less specificity. Nevertheless, PSAP complements PSA in the right clinical context.

Composition: Anti-human Prostatic Acid Phosphatase mouse monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide

Name: J Chain Immunoglobulin Antibody Clone SP105

Description and applications: J chain is a small glycopeptide and is structurally unrelated to heavy or light chains, but is synthesized by all plasma cells that secrete polymeric immunoglobulins. J chain is linked to IgA and IgM by disulfide bonds, however, it has been detected in IgG- and IgD-containing cells. J chains are present in a large proportion of the immunoglobulin-positive cells in the germinal centers of the tonsil and lymph node. B cells secrete J chain at an early stage of differentiation with the expression persisting in those cells destined to produce IgA or IgM. It has been shown that a significant proportion of mesangial immunoglobulin deposits of IgA nephropathy are dimeric and therefore positive for this antibody.

Composition: anti-human J Chain immunoglobulin rabbit monoclonal antibody purified and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Name: IMP 3 Antibody Clone EP286

Description and applications: IMP-3, known as Insulin-like growth factor 2 (IGF-II) mRNA-binding protein 3, is an oncofetal protein that stabilizes IGF-II mRNA for trafficking and plays an important role in cell growth and migration. This 65- 70 kDa protein is expressed normally in developing tissues during early embryogenesis in a variety of fetal tissues including the liver, lung kidney, thymus, and placenta, but at low or undetectable levels in normal adult tissues. Recent studies have demonstrated IMP-3 expression in various malignant tumors of the lung, gastrointestinal tract, liver, endometrium, and bladder, while undetectable in adjacent benign tissues. IMP-3 may have a critical role in tumor proliferation, invasion and metastasis, and has been suggested to be an independent marker for poor prognosis in patients with clear cell carcinomas.

Composition: Anti-human IMP 3 rabbit monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Name: Inhibin Alpha Antibody (Clone R1)

Description and applications: This antibody recognizes the 32kDa alpha subunit of human inhibin. Inhibin alpha is expressed in a range of tissues including prostate, brain, adrenal gland, testis and ovary. The antibody may be of value in the differentiation of adrenocortical tumors, placental and gestational trophoblastic lesions and sex cord stromal tumors.

Composition: anti-human Inhibin alpha mouse monoclonal antibody purified from ascites. Prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Name: Rabbit Anti-Human Ki-67 Monoclonal Antibody clone SP6

Description and aplications: This rabbit monoclonal antibody (clone SP6) reacts with a nuclear antigen which is expressed in every proliferating human cell. It is expressed mainly during the cell cycle phases, including late G1, S, G2 and M phase. The cells in the G0 phase (quiescent cells) are not immunostained. This antibody is useful to identify the degree of cell proliferation. Is a marker to define the growth fraction in benign and malignant tissues, such as prostate, breast or lymphoid tissues. Therefore, it can play an important role as a predictor of malignancy.

Composition: anti-Ki67 rabbit monoclonal antibody obtained from supernatant culture and prediluted in a tris buffered solution pH 7.4 containing 0.375mM sodium azide solution as bacteriostatic and bactericidal.

Intended use : Immunohistochemistry (IHC) on paraffin embedded tissues. Not tested on frozen tissues or Western-Blotting

Immunogen: Synthetic peptide derived from the Cterminus of human Ki67 protein

Species reactivity: In vitro diagnostics in humans. Not tested in other species

Name: Keratin 18 Antibody Clone DC-10

Description and applications: The nomenclature coined in 1982 by Moll and Franke assigned ranges from 1 to 8 for keratin type II (neutral or basic) and between 9 and 21 for type I (acidic). However and because of the high homology among the different molecules is common for a single monoclonal antibody to react with different types of keratins , for example keratin brand AE1 keratins 10, 14 , 15, 16 and 19. This antibody reacts with the acid intermediate filament protein of 45 kDa keratin, identified as Keratin 18. Keratin 18 is generally co-expressed with the keratin 8 in most simple gland epithelia. In general, the keratin 18 does not react with squamous multilayered epithelium. In neoplastic tissues, this antibody reacts with different benign and malignant epithelial lesions. Most adenocarcinomas and basal cell carcinomas express this keratin, whereas it is absent in squamous cell carcinomas.

Composition: anti-Keratin 18 mouse monoclonal antibody obtained from supernatant culture and prediluted in a tris buffered solution pH 7.4 containing 0.375mM sodium azide solution as bacteriostatic and bactericidal. The amount of the active antibody in the sample is 3,3 μg/mL..

Immunogen: Human breast cancer PMC 42 cells.

Name: LIN-28 Antibody Clone EP150

Description and applications: LIN28 is a highly conserved, RNA-binding protein (RBP). It plays an important role as a translational enhancer, leading specific mRNAs to polysomes and therefore increasing the competence of protein synthesis. LIN28 was identified as a negative regulator of miRNA biogenesis and suggested to play a central role in blocking miRNAmediated differentiation in stem cells and certain cancers. LIN28 is expressed by various undifferentiated embryonic cell types. Anti- LIN28 has been used as a sensitive marker for germ cell tumors. The positive staining of LIN28 in yolk sac tumors showed an advantage over OCT4, which is negative in these tumors.

Composition: Anti-human LIN-28 rabbit monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Name: Rabbit anti-human Myo-D1 Monoclonal Antibody clone EP212

Description and applications: MyoD1 is a protein with a key role in regulating muscle differentiation. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. The protein is also involved in muscle regeneration. MyoD1 is expressed in developing skeletal muscle tissue but faintly in adult skeletal muscle. In abnormal tissues, it labels tumour cell in rhabdomyosarcoma. MyoD1 is one of the earliest markers of myogenic commitment. Antibody to MyoD1 has been useful to differentiate rhabdomyosarcomas from other tumors. It is a sensitive and specific marker for myogenic differentiation

Composition: anti-human Myo-D1 rabbit monoclonal antibody purified from ascites. Prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide

Intended use: Immunohistochemistry (IHC) on paraffin embedded tissues. Not tested on frozen tissues or Western-Blotting

Name: Mast Cell Tryptase Antibody Clone EP259

Description and applications: Human mast cell tryptase, comprises a family of trypsin-like neutral serine proteinases that are predominantly expressed in mast cells. Tryptase has effects on peptides, proteins, cells, and tissues, and many of these actions can ultimately contribute to asthma symptoms. Mast-cell tryptase is found in mast-cell granules and has also been reported to be expressed by peripheral blood basophils at low level. Tryptase has been used as a marker of mast cell activation.

Composition: Anti-human Mast Cell Tryptase rabbit monoclonal antibody purified from serum and prepared in 10mM
PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.