مونوکلونال

Name: CD33 Monoclonal Antibody (Clone PWS44)

Description and applications: Clone PWS44 detects the CD33 antigen on the membrane and in the cytoplasm of cells of myelomonocytic lineages. In normal bone marrow trephine biopsies, clone PWS44 stains myeloid and myelomonocytic hemopoiesis and mature macrophages; cells of the erythroid and megakaryocytic series are negative. Some reactivity was observed in cytotrophoblasts and syncytiotrophoblasts of placenta. Except for dendritic cells and infiltrating macrophages, no staining was observed in other normal tissues evaluated. Clone PWS44 stained acute myeloid leukemias, chronic myeloid leukemia, granulocytic sarcomas and cases of myeloid dysplasia and myeloproliferative diseases. PWS44 stained cases of non-lymphoid tumours. In the lymphoid neoplasms tested CD33 staining was observed in precursor B-lymphoblastic leukemias, precursor T-lymphoblastic leukemias, classical Hodgkin’s lymphomas, Burkitt lymphomas, plasma cell neoplasms, hematodermic lymphoid neoplasms, anaplastic large cell lymphoma, lymphoplasmacytic lymphoma.

Composition: anti-human CD33 mouse monoclonal antibody purified from tissue culture supernatant. Prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Immunogen: Prokaryotic recombinant protein corresponding to a region of the C2 domain of the human CD33 molecule.

Name: CD42b (42C01)

Description and applications: The CD42b glycoprotein, also known as GPIb, is a co-factor of ristocetin-induced aggregation and is involved in the binding of platelets to blood vessel walls. The CD42b antigen is expressed on platelets and on megakaryocytes in bone marrow. The absence of CD42b antigen on platelets may indicate Bernard-Soulier disease. The antibody is of value in the immunophenotyping of megakaryoblastic leukaemias that express one or more markers associated with platelets (CD41, CD61 and CD42b). In chronic myeloproliferative processes, like chronic idiopathic myelofibrosis in prefibrotic stage besides appearing in greater numbers the megakaryocytes are markedly abnormal while megakaryocytes in unclassifiable myelodysplastic / myeloproliferative diseases there is an increased of normal or small megakaryocytes (micromegakaryocytes).

Composition: anti-human CD42b mouse monoclonal antibody purified from the ascites fluid by Protein G chromatography. Prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Immunogen: Recombinant CD42b.

Name: CD61 Antibody (Clone 2F2)

Description and aplications: CD61 (GPIIIa) is a glycoprotein found on megakaryocytes, platelets and their precursors. CD61 antigen plays a role in platelet aggregation and also as a receptor for fibrinogen, fibronectin, von Willebrand factor and vitronectrin. This antibody is useful in detecting platelet precursors neoplasia, normal platelets and the majority of the megakaryocytic leukemia cases.

Composition: anti-human CD61 mouse monoclonal antibody purified from ascites. Prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Immunogen: Recombinant protein encoding part of the external domain of human CD61.

Name: COX2 (Cyclooxygenase 2) Antibody (Clone SP21)

Description and applications: COX-2 (Cyclooxygenase-2) is an inducible enzyme. It is involved in the response of cells to growth factors, tumor promoters, and cytokines that induce its expression. Given its role in synthesizing prostaglandins, COX-2 is therefore of interest in studying immune response regulation. COX-2 is induced by a wide variety of stimuli and was initially identified as immediate-early growth response gene. In addition, COX-2 expression markedly increased in 85-90% of human colorectal adenocarcinoma whereas COX-1 levels remain unchanged. COX-2 is expressed in normal cells from mammary acini, uterine cervix, uterus, fallopian tubes, trophoblast of chorionic villi, prostate, seminal vesicles, epididymis, bladder urothelial epithelium, gastric glands or gallbladder. Consequently, COX-2 is expressed in many solid tumors. Intense to moderate cytoplasmic and sometimes membrane expression is observed in colorectal, prostate, cervical, endometrial, ovarian, urothelial, pancreatic and hepatic carcinomas. COX-2 is frequently expressed in breast carcinomas while up to 80% of pituitary adenomas are positive. The prognostic value of COX-2 expression is controversial.

Composition: anti-human COX2 rabbit monoclonal antibody purified by protein affinity and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Immunogen: Synthetic peptide corresponding to C-terminus of rat COX-2.

Name: INI-1 (BAF47/SNF5) Antibody (Clone 25/BAF47)

Description and applications: SWI/SNF complexes facilitate gene activation and transcription factor binding by altering repressive chromatin structures in an ATP-dependent manner. In mammals, SWI/SNF complexes are present in mutliple forms that include 9-12 BRG1-associated factors (BAFs) per complex. These BAF proteins range in molecular weight from 47 to 250 kDa. BAF47/SNF5 (SMARCB1/Ini1) complexes with BRG1- and BRM-containing SWI/SNF complexes. BAF47/SNF5 mRNA is widely expressed in cell lines and tissues, and BAF47/SNF5 gene is mutated in many human tumors. The latter is indicative of a tumor suppressor role for BAF47/SNF5. In addition, mice deficient for BAF47 die early in embryogenesis, while BAF47/SNF5 heterozygous mice display a variety of tumors in the soft tissues of the head and neck. BAF47/SNF5 also binds the HIV-1 integrase and stimulates integrase-mediated DNA joining activity. Thus, BAF47/SNF5 is a component of SWI/SNF complexes that may be critical for normal development and tumor suppression, but may also be a protein utilized for viral DNA integration into host DNA. Expression in normal tissues is ubiquitous. In tumor tissue loss of protein expression SMARCB1 / INI1 gene was first observed in all malignant rhabdoid tumors of childhood of different location (soft tissue, kidney and CNS) and in medullary carcinomas of the kidney. Also loss of expression is observed in 50% of synovial sarcomas, some myxoid chondrosarcoma and myoepithelial carcinomas, almost all epithelioid sarcoma (proximal and conventional variants) and 50% of malignant tumors of peripheral nerves sheat, all presenting rhabdoid morphology. Loss of immunohistochemistry expression is also observed in cases of poorly differentiated chordoma. The antibody is useful in differentiating rhabdoid tumors of other tumors with similar morphology as desmoplastic round cell tumor, rhabdomyosarcoma and Wilms tumor do not show loss of expression of INI1. The antibody also is useful in confirming the diagnosis of medullary carcinoma and discard possible renal pelvis urothelial carcinoma or other carcinomas of the kidney showing expression for INI1. Maintained staining in most carcinomas, epithelioid angiosarcoma, mesotheliomas, melanomas and other tumors or lesions reagents (granulomas) of epithelioid morphology make this useful antibody in the diagnosis of epithelioid sarcoma.

Composition: anti-human INI-1 mouse monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Immunogen: mouse BAF47 aminoacids 257-359.

Name: Arginase-1 Antibody (Clone EP261)

Description and applications: Arginase is a manganese metalloenzyme that catalyzes the hydrolysis of arginine to generate ornithine and urea. Arginiase I and II are isoenzymes which differ in subcellular localization, regulation, and possibly function. Arginase I is a cytosolic enzyme, which is expressed mainly in the liver as part of the urea cycle, whereas arginase II is a mitochondrial protein found in a variety of tissues. Antibody to ARG-1 labels hepatocytes in normal tissues and granulocytes in peripheral blood. ARG-1 is a sensitive and specific marker for identification of hepatocellular carcinoma. This antibody is very useful in distinguishing between: 1) liver metastases of various adenocarcinomas and hepatocellular carcinoma (HCC), which can be a realdiagnostic challenge, especially in small biopsies or material from fine needle aspiration ( FNA) and 2) the distinction between different histological variants of HCC and cholangiocarcinoma. Specifically, the ARG-1 antibody is key in the diagnosis of scirrhous hepatocellular carcinoma, where specific markers for adenocarcinomas are generally positive while HepPar-1 in some cases it may be negative. In addition, several studies have shown that ARG-1 antibody is more sensitive than HepPar-1 for immunohistochemical diagnosis of hepatocellular carcinoma while the second is usually negative in poorly differentiated hepatocellular carcinomas and can be positive in adenocarcinomas of pancreatic, gastric, colic origin or even cholangiocarcinoma. However, isolated cases of pancreatic adenocarcinomas and cholangiocarcinomas presented focal staining against ARG-1. In this line, it should be considered that the ARG-1 antibody does not allow differential diagnosis between benign, dysplastic and malignant hepatocyte lesions.

Composition: anti-human ARG-1 rabbit monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Name: CD10 Antibody (Clone 56C6)

Description and aplications: CD10, also known as Common Acute Lymphocytic Leukemia Antigen (CALLA), is a cell surface enzyme with neutral metalloendopeptidase activity which inactivates a variety of biologically active peptides. CD10 is expressed on the cells of lymphoblastic, Burkitt’s, and follicular germinal center lymphomas, and on cells from patients with chronic myelocytic leukemia (CML). It is also expressed on the surface of normal early lymphoid progenitor cells, immature B cells within adult bone marrow and germinal center B cells within lymphoid tissue. CD10 is also present on breast myoepithelial cells, bile canaliculi, fibroblasts, with especially high expression on the brush border of kidney and gut epithelial cells.

Composition: anti-human CD10 mouse monoclonal antibody purified from ascites fluid by Protein A chromatography. Prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Name: CD103  Antibody (Clone EP206)

Description and applications: CD103, also known as integrin alpha E (ITGAE), is an integrin protein that in humans is encoded by the ITGAE gene. It binds integrin beta 7 to form the complete heterodimeric molecular αEβ7 that binds to an extracellular matrix component and cellular counter receptor. They mediate cell adhesion, migration and signaling and are important for T lymphocyte localization. CD103 is expressed on intraepithelial lymphocytes in mucosal areas, including lung and GI tract. In malignancies, CD103 is present on all enteropathy-type T-cell lymphomas. Additionally, CD103 has been a useful marker for hairy cell leukemia.

Composition: anti-human CD130 rabbit monoclonal antibody purified from ascites. Prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Name: CD11c (Integrin Alpha-X) Antibody (Clone EP157)

Description and applications: CD11c (ITGAX), a member of the leukointegrin family, shares the same beta subunit with other members of the leukocyte adhesion molecule family, which includes CD11a (LFA-1), CD11b (MAC-1) and CD11d (ITGAD), but has a unique alpha chain. CD11c has been shown to play a role in phagocytosis, cell migration, and cytokine production by monocytes/macrophages as well as induction of T cell proliferation by Langerhans cells. CD11c is expressed prominently on the plasma membranes of monocytes, tissue macrophages, NK cells, and most dendritic cells (DCs). A lower level of expression is also observed on neutrophils as a result of its high level of expression on most DCs. An antibody to CD11c may aid in identification of lesions with histiocytic origin. It may also been used as a marker for hairy cell leukaemia in paraffin embedded tissues.

Composition: anti-human CD11c rabbit monoclonal antibody purified from serum and prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Name: Rabbit anti-human CD13 Monoclonal Antibody

Composition: anti-human CD13 rabbit monoclonal antibody purified from ascites fluid by chromatography. Prepared in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide

Intended use: Immunohistochemistry (IHC) on paraffin embedded tissues. Not tested on frozen tissues or Western-Blotting

Immunogen: A synthetic peptide corresponding to residues in human CD13 protein

Visualization: Cell membrane

Name:CD138 Antibody clone EP201

Description and applications: CD138, also known as Syndecan-1, is a member of the transmembrane heparan sulfate proteoglycan family, acts as an extracellular matrix receptor and is involved in many cellular functions, including cell-cell adhesion and cell-matrix adhesion. CD138 expression is found in both hematopoietic and non-hematopoietic cells. In the hematopoietic system, CD138 labels plasma cells. It is an excellent marker for plasmacytic differentiation within the spectrum of hematologic malignancy. It´s a very usefull tool in the diagnostic of chronic endometritis associated with infertility. Among nonhematolymphoid cells, CD138 reactivity is observed inmany types of epithelial cells and stoma cells in both normal and tumor tissues. These staining should
be remembered and not interpreted as unspecific.

Composition:Anti-human CD138 rabbit monoclonal antibody purified from serum and prepared in 10mM PBS, pH
7.4, with 0.2% BSA and 0.09% sodium azide

Intended use: Immunohistochemistry (IHC) on paraffin embedded tissues. Not tested on frozen tissues or Western-Blotting