دسته: پلی کلونال
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آنتی بادیهای ایمونوهیستوشیمیآنتی بادی TDT کلون Polyclonal برند Patho Sage
Specification:
TDT is a DNA polymerase that catalyses the addition of deoxynucleotides to free 3’OH groups on polydeoxynucleotide chains. TDT is present in the nucleoli of normal T and B lymphocyte precursors and the neoplastic equivalent. Positive cells are most abundant in normal thymus, especially in the cortex, with little or no labelling of the medulla. A few cells in the normal bone marrow corresponding to hematopoietic precursor cells, also express TDT. TDT is a valuable marker in the identification of immature T and B Lymphocyte precursors and acute leukaemia’s. It is expressed at high level in T cell and pre-B cell acute lymphoblastic leukaemia’s and lymphomas. B-cell ALL and mature (or peripheral) B and T cell malignancies are TDT-negative. TDT may also be expressed in some cases of acute myeloid leukaemia.
Presentation:
Rabbit anti-calf TDT, 100-200 μg/ml immuno-affinity purified, solved in 1% BSA in Phosphate buffered saline with 0.1% sodium azide in PBS as preservative
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آنتی بادیهای ایمونوهیستوشیمی
آنتی بادی CD3 کلون Polyclonal برند Patho Sage
Specification:
Anti-CD3 antibody has been considered the best all around T-cell marker. This antibody reacts with an antigen present in early thymocytes. The positive staining of this marker may represent a sign of early commitment to the T-cell lineage.
Presentation:
Anti-CD3 is a rabbit polyclonal antibody purified from rabbit anti-sera diluted in tris buffered saline, pH 7.37.7, with protein base, and preserved with sodium azide.
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آنتی بادیهای ایمونوهیستوشیمی
آنتی بادی SOX10 کلون Polyclonal برند Patho Sage
Specification:
Sry-related HMG-BOX gene 10 (SOX-10) is a nuclear transcription factor that participates in neural crest development and in the specification and differentiation of cells of melanocytic lineage. It has been recently shown to be a sensitive marker of melanoma, including conventional, spindled, and desmoplastic subtypes. Anti-SOX-10 antibody was applied to a variety of neural crest-derived tumors, mesenchymal and epithelial neoplasms, and normal tissues. SOX-10 nuclear expression was found in 76 of 78 melanomas (97%) and 38 of 77 malignant peripheral nerve sheath tumors (49%), whereas S100 protein was expressed in 71 melanomas (91%) and 23 malignant peripheral nerve sheath tumors (30%). SOX-10 was expressed by metastatic melanomas and nodal capsular nevus in sentinel lymph nodes, but not by other lymph node components such as dendritic cells which usually express S100 protein. In scar specimens, immature fibroblasts, epithelioid granulomas, and histiocytic proliferations can histo-pathologically mimic residual melanoma and even be positive for MITF and S100. However, SOX-10 is less likely to be expressed by fibroblasts or histiocytes, especially compared to MITF and S100. Anti-SOX-10 produces a nuclear stain that provides a clean signal that is much sharper and darker in staining quality when compared to the use of antibodies against MITF and S100. The nuclear signal provided by both anti-SOX-10 and anti- MITF was easier to interpret than cytoplasmic stains, such as anti-S100 (nuclear and cytoplasmic), anti-HMB-45, and anti-Melan-A (both cytoplasmic), especially in the intraepidermal component where these cytoplasmic markers can non-specifically adhere to melanin. Therefore, anti-SOX-10 has been shown to be superior to all other immunostains in detecting residual invasive and in situ melanoma. Anti-SOX-10 is also a useful marker in detecting both the in situ and invasive components of desmoplastic melanoma. It is known that the commonly used melanoma markers, anti-HMB-45 and anti-Melan-A, are poorly expressed in desmoplastic melanomas while it has been reported that anti-SOX-10 was moderately to strongly expressed in all 28 desmoplastic melanomas tested. As an intraepidermal component that is frequently present in greater than 50% of desmoplastic melanomas, the presence of SOX-10 may prove its usefulness over MITF in the evaluation of melanoma in situ, as it is much more likely to be expressed by a subtle, underlying desmoplastic melanoma than MITF. SOX10 is strongly expressed by melanoma cells and is not or very weakly expressed by the spindle fibroblasts in scar. These findings underscore the utility of anti- SOX-10 in the differential diagnosis of residual desmoplastic melanoma versus scar and show the clinical value of anti-SOX-10 in the evaluation of melanoma re-excision specimens. SOX-10 is diffusely expressed in schwannomas and neurofibromas. SOX-10 presence was not identified in any other mesenchymal and epithelial tumors except for myoepitheliomas and diffuse astrocytomas. SOX-10 expression is seen in sustentacular cells of pheochromocytomas and paragangliomas, and occasionally carcinoid tumors from various organs, but is not seen in the tumor cells. In normal tissues, SOX-10 is expressed in Schwann cells, melanocytes, and myoepithelial cells of salivary, bronchial, eccrine, and mammary glands. SOX-10 expression is also observed in mast cells in a variety of tissues and organs in both nuclear and cytoplasmic reaction.
Presentation:
Concentrated antibody in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide
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آنتی بادیهای ایمونوهیستوشیمی
آنتی بادی Alpha1 Antitrypsin کلون Polyclonal برند Patho Sage
Specification:
The immunohistochemical staining of Alpha-1-Antitrypsin is very useful in the study of inherited AAT deficiency, benign and malignant hepatic tumors and yolk sac carcinomas. Positive staining for A-1-Antitrypsin may also be used in detection of benign and malignant lesions of a histiocytic nature. Sensitivity and specificity of the results have made this antibody a useful tool in the screening of patients with cryptogenic cirrhosis or other forms of liver disease with portal fibrosis of uncertain etiology.
Presentation:
Anti-A-1-Antitrypsin is a rabbit polyclonal antibody purified from rabbit anti-sera diluted in tris buffered saline, pH 7.3-7.7, with protein base, and preserved with sodium azide.
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آنتی بادیهای ایمونوهیستوشیمی
آنتی بادی ATRX کلون Polyclonal برند Patho Sage
Specification:
Defects in alpha thalassemia/mental retardation syndrome X-linked (ATRX), a gene that encodes a protein involved in chromatin remodeling. ATRX mutation is a marker of astrocytic lineage among the IDH1-mutant gliomas and is mutually exclusive with 1p/19q codeletion. ATRX mutations are most frequently in grade II (67%) and grade III (73%) astrocytomas and secondary glioblastoma multiforme (GBM) (75%), whereas they are uncommon in primary GBMs and oligodendrogliomas. Nearly all diffuse gliomas with IDH and ATRXmutations also harbor TP53 mutation and are associated with the alternative lengthening of telomeres (ALT) phenotype. ATRX mutation also occurs in many other types of human tumors, such as neuroendocrine tumors. Immunohistochemistry for ATRX demonstrates a loss of protein expression in neoplastic cells that harbor inactivating mutations, whereas expression is retained in nonneoplastic cells within the sample, such as endothelial cells.
Immunogen:
Synthetic peptide from human ATRX protein
Presentation:
Purified antibody fraction from rabbit anti-serum with 0.2% BSA and 15mM sodium azide