Name: Flow Cytometry Antibody CD14-PE, Clone MEM-15
- Antibody CD14-PE is a monoclonal antibody (mAb) labelled with R-phycoerythrin (PE) designed for flow cytometry (FC) use as a direct immunofluorescence reagent in the identification and enumeration of CD14 antigen-expressing cells. This reagent must be used by flow cytometry qualified personal.
SUMMARY AND EXPLANATION
Leukocytes are named according to structure as being either granulocytes or agranulocytes, and according to the function as either phagocytes or immunocyte. The granulocytes, which include neutrophils, basophils and eosinophils, are all phagocytes. Of the agranulocytes, the monocytes and macrophages are phagocytes whereas the lymphocytes are immunocytes. Monocytes ingest dead or damaged cells and help defend against many infectious organisms. CYT-14PE7 mAb recognizes CD14 antigen present on monocyte/macrophage cells. Anti-CD14 is important for excluding CD14+ monocytes in relation to T lymphocytes subsets analysis. In flow cytometry, anti-CD14 is considered essential for the initial evaluation of acute myeloid leukaemias together with a panel of other antibodies.
- The purified monoclonal CD14 antibody conjugated with R-phycoerythrin (PE) is supplied in phosphate-buffered saline (PBS) containing 0.09% sodium azide.
- Clone: MEM-15.
- Isotype: IgG1.
- Amount per 1 mL vial: 200 tests (5 µL mAb per determination).
- Reagent is considered non-sterile.