Name: Flow Cytometry Antibody CD16-PE, Clone 3G8
- Antibody CD16-PE is a monoclonal antibody (mAb) labelled with R-phycoerythrin (PE) designed for use as a direct immunofluorescence reagent in the identification and enumeration of cells which express the CD16 antigen by flow cytometry.
SUMMARY AND EXPLANATION
Flow Cytometry is a powerful tool for the analytical and quantitative characterization of cells which provides rapid, quantitative and multiparametric analysis of heterogeneous cell populations on a cell-by-cell basis. Flow cytometry is performed on cells in liquid suspension that have been incubated with fluorescently labeled antibodies directed against specific cellular proteins. The relative fluorescence intensity of the positive cells indicates the amount of antibody bound to specific binding sites on the cells, and therefore provides a relative measure of antigen expression. Human lymphocytes may be classified in three main populations according to their biological function and their cell surface antigen expression: T lymphocytes, B lymphocytes and natural killer cells (NK). NK cells (CD3-CD16+) mediate cytotoxicity against certain tumors and virus-infected cells. NK-mediated cytotoxicity does not require class I or class II major histocompatibility complex (MHC) molecules to be present on the target cells. The CYT-16PE7 mAb recognizes the CD16 antigen present on NK cells, macrophages, and neutrophils, and can therefore be used in the characterization studies for immunophenotyping of leucocytes. These studies are widely applied for monitoring of the immunologic status of post-transplant patients and in the characterization and follow-up of immunodeficiencies, autoimmune diseases, leukemia etc.
Purified monoclonal CD16 Antibody conjugated with R-phycoerythrin (PE), supplied in phosphate buffered saline with 0,1% sodium azide.
- Clone: 3G8
- Isotype: IgG1
- Purification: Affinity chromatography
- Amount per 1 ml vial: 200 tests (5 µl mAb to 106 cells)
- Reagents are not considered sterile.