Name: Flow Cytometry Antibody CD20-PE, Clone Bly1
- Antibody Anti-human CD20 reagent is a monoclonal antibody (mAb) conjugated with different fluorochromes (see table) and designed for use as a direct immunofluorescence reagent in the identification and enumeration of cells which express the CD20 antigen by Flow Cytometry (FC). This reagent must be used by FC qualified personal. CD20 can be considered a pan-B cell antigen, as it is expressed on the surface of all mature B-lymphocytes but not in secreting plasma cells.
SUMMARY AND EXPLANATION
FC is a powerful tool in analytical and quantitative characterization of cells which provides rapid and multiparametric analysis of heterogeneous cell populations on a cell-by-cell basis. Flow cytometry is performed on cell suspension after incubating it with fluorescentlabelled antibodies directed against specific cellular proteins. Positive cells relative fluorescence intensity indicates the amount of antibody bonded to specific cell sites providing information about antigen expression. Human lymphocytes may be classified in three main populations according to their biological function and their cell surface antigen expression (T lymphocytes, B lymphocytes and natural killer (NK) cells). B lymphocytes are the producers of antibodies, they mediate humoral immunity particularly effective against toxins, whole bacteria, and free viruses. Anti-human CD20 reagent recognizes CD20 antigen present on B-cells,and can therefore be used in lymphocytes immunophenotyping. Antibodies to CD20 are widely applied in the characterization and follow-up of acute and chronic lymphoproliferative disorders.
Purified anti-human CD20 mAb conjugated with different fluorochromes (see table above) is supplied in phosphate-buffered saline (PBS) containing ≤0,09% (m/v) sodium azide
- Amount per vial: 200 tests (5 µl mAb per determination) (see table above).
- Reagent is considered non-sterile.