Name: Flow Cytometry Antibody CD23-FITC, Clone D3.1
- CD23-FITC is a monoclonal antibody (mAb) labelled with Fluorescein isothiocyanate (FITC) designed for flow cytometry (FC) use as a direct immunofluorescence reagent in the identification and enumeration of CD23 antigen-expressing cells.
SUMMARY AND EXPLANATION
Human lymphocytes may be classified in three main populations according to their biological function and their cell surface antigen expression (T lymphocytes, B lymphocytes and natural killer (NK) cells). B lymphocytes are the producers of antibodies, they mediate humoral immunity particularly effective against toxins, whole bacteria, and free viruses. CD20 can be considered a pan-B cell antigen, as it is expressed on the surface of all mature B-lymphocytes but not in secreting plasma cells. Anti-human CD20 reagent recognizes CD20 antigen present on B cells and can therefore be used in lymphocytes immunophenotyping. Antibodies to CD20 are widely applied in the characterization and follow-up of acute and chronic lymphoproliferative disorders. Pacific BlueTM is excited with the violet laser (405nm) and emits at 550nm. This fluorochrome provides maximum resolution and narrow emission peaks, which results in little spectral overlap and minimal compensation requirements. In multicolor panel it is recommended the use of this fluorochrome combined with Orange Cytognos 515, which is also excited with the violet laser and emits at 515nm.
The purified monoclonal CD23 Antibody conjugated with Fluorescein Isothiocyanate (FITC) is supplied in phosphate-buffered saline (PBS) containing 0,09% sodium azide.
- Clone: D3.1
- Isotype: IgG2a.
- Amount per 1 ml vial: 200 tests (5 µl mAb per determination).
- Reagent is considered non-sterile.